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XB-ART-4637
Development November 1, 2003; 130 (22): 5425-36.

GBP binds kinesin light chain and translocates during cortical rotation in Xenopus eggs.

Weaver C , Farr GH , Pan W , Rowning BA , Wang J , Mao J , Wu D , Li L , Larabell CA , Kimelman D .


Abstract
In Xenopus, axis development is initiated by dorsally elevated levels of cytoplasmic beta-catenin, an intracellular factor regulated by GSK3 kinase activity. Upon fertilization, factors that increase beta-catenin stability are translocated to the prospective dorsal side of the embryo in a microtubule-dependent process. However, neither the identity of these factors nor the mechanism of their movement is understood. Here, we show that the GSK3 inhibitory protein GBP/Frat binds kinesin light chain (KLC), a component of the microtubule motor kinesin. Upon egg activation, GBP-GFP and KLC-GFP form particles and exhibit directed translocation. KLC, through a previously uncharacterized conserved domain, binds a region of GBP that is required for GBP translocation and for GSK3 binding, and competes with GSK3 for GBP. We propose a model in which conventional kinesin transports a GBP-containing complex to the future dorsal side, where GBP dissociates and contributes to the local stabilization of beta-catenin by binding and inhibiting GSK3.

PubMed ID: 14507779
Article link: Development
Grant support: [+]
Genes referenced: dvl1 dvl2 frat1 gsk3b kif5b klc1 myc pias1 tpr


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