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XB-ART-46403
Dev Biol July 1, 2013; 379 (1): 16-27.

The Xenopus Tgfbi is required for embryogenesis through regulation of canonical Wnt signalling.

Wang F , Hu W , Xian J , Ohnuma S , Brenton JD .


Abstract
Tgfbi, a fasciclin family extracellular matrix protein, has various roles in human diseases from corneal dystrophies to cancer. However, the molecular mechanisms that underlie its functions are poorly understood. Here, we studied the role of Tgfbi during Xenopus embryogenesis. During gastrulation and immediately after, Xtgfbi is expressed at developmentally important signaling centers including the dorsal marginal zone, notochord and floorplate. Xtgfbi knockdown by anti-sense morpholinos causes defective organizer induction, patterning and differentiation of muscle, neuron and neural crests, similar to suppression of canonical Wnt signaling. In Xenopus embryos and animal caps as well as DLD-1 cells, we show that Tgfbi is strongly required for the full activation of the canonical Wnt pathway by promoting phosphorylation of GSK3β and consequently enhancing the stabilization and nuclear localization of β-catenin. Further analysis shows that Tgfbi is likely to promote GSK3β phosphorylation through integrin-linked kinase.

PubMed ID: 23246513
Article link: Dev Biol
Grant support: [+]
Genes referenced: axin1 cdh1 chrd.1 ctnnb1 dlc dld dvl2 egr2 en2 gal.2 gsc gsk3b lef1 lrp6 mix1 mixer myh4 myh6 myod1 not rho.2 slc12a3 sox2 tgfbi tubb2b twist1
GO keywords: eye development [+]
Morpholinos: tgfbi MO1 tgfbi MO2

Disease Ontology terms: corneal dystrophy

Article Images: [+] show captions


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