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J Biol Chem
2012 Oct 05;28741:34325-36. doi: 10.1074/jbc.M112.400192.
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Cohesin acetylation promotes sister chromatid cohesion only in association with the replication machinery.
Song J
,
Lafont A
,
Chen J
,
Wu FM
,
Shirahige K
,
Rankin S
.
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Acetylation of the Smc3 subunit of cohesin is essential to establish functional cohesion between sister chromatids. Smc3 acetylation is catalyzed by members of the Eco family of acetyltransferases, although the mechanism by which acetylation is regulated and how it promotes cohesion are largely unknown. In vertebrates, the cohesin complex binds to chromatin during mitotic exit and is converted to a functional form during or shortly after DNA replication. The conserved proliferating cell nuclear antigen-interacting protein box motif in yeast Eco1 is required for function, and cohesin is acetylated during the S phase. This has led to the notion that acetylation of cohesin is stimulated by interaction of Eco1 with the replication machinery. Here we show that in vertebrates Smc3 acetylation occurs independently of DNA replication. Smc3 is readily acetylated before replication is initiated and after DNA replication is complete. However, we also show that functional acetylation occurs only in association with the replication machinery: disruption of the interaction between XEco2 and proliferating cell nuclear antigen prevents cohesion establishment while having little impact on the overall levels of Smc3 acetylation. These results demonstrate that Smc3 acetylation can occur throughout interphase but that only acetylation in association with the replication fork promotes sister chromatid cohesion. These data reveal how the generation of cohesion is limited to the appropriate time and place during the cell cycle and provide insight into the mechanism by which acetylation ensures cohesion.
Beckouët,
An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion.
2010, Pubmed
Beckouët,
An Smc3 acetylation cycle is essential for establishment of sister chromatid cohesion.
2010,
Pubmed
Blow,
Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.
1986,
Pubmed
,
Xenbase
Borges,
Hos1 deacetylates Smc3 to close the cohesin acetylation cycle.
2010,
Pubmed
Dreier,
Regulation of sororin by Cdk1-mediated phosphorylation.
2011,
Pubmed
Funabiki,
The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement.
2000,
Pubmed
,
Xenbase
Gandhi,
Human Wapl is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase.
2006,
Pubmed
Gillespie,
Scc2 couples replication licensing to sister chromatid cohesion in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Grosse,
The primase activity of DNA polymerase alpha from calf thymus.
1985,
Pubmed
Guacci,
A direct link between sister chromatid cohesion and chromosome condensation revealed through the analysis of MCD1 in S. cerevisiae.
1997,
Pubmed
Guacci,
Cohesin-independent segregation of sister chromatids in budding yeast.
2012,
Pubmed
Heidinger-Pauli,
Distinct targets of the Eco1 acetyltransferase modulate cohesion in S phase and in response to DNA damage.
2009,
Pubmed
Heidinger-Pauli,
Genetic evidence that the acetylation of the Smc3p subunit of cohesin modulates its ATP-bound state to promote cohesion establishment in Saccharomyces cerevisiae.
2010,
Pubmed
Higashi,
The prereplication complex recruits XEco2 to chromatin to promote cohesin acetylation in Xenopus egg extracts.
2012,
Pubmed
,
Xenbase
Hou,
Two human orthologues of Eco1/Ctf7 acetyltransferases are both required for proper sister-chromatid cohesion.
2005,
Pubmed
Kueng,
Wapl controls the dynamic association of cohesin with chromatin.
2006,
Pubmed
Lafont,
Sororin cooperates with the acetyltransferase Eco2 to ensure DNA replication-dependent sister chromatid cohesion.
2010,
Pubmed
,
Xenbase
Losada,
Identification of Xenopus SMC protein complexes required for sister chromatid cohesion.
1998,
Pubmed
,
Xenbase
Luke,
Quantitation of type II topoisomerase in oocytes and eggs of Xenopus laevis.
1989,
Pubmed
,
Xenbase
Lyons,
Cdk1-dependent destruction of Eco1 prevents cohesion establishment after S phase.
2011,
Pubmed
Maddox,
Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics.
2003,
Pubmed
,
Xenbase
Mimura,
Central role for cdc45 in establishing an initiation complex of DNA replication in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
Moldovan,
PCNA controls establishment of sister chromatid cohesion during S phase.
2006,
Pubmed
Mönnich,
A zebrafish model of Roberts syndrome reveals that Esco2 depletion interferes with development by disrupting the cell cycle.
2011,
Pubmed
Nishiyama,
Sororin mediates sister chromatid cohesion by antagonizing Wapl.
2010,
Pubmed
,
Xenbase
Rankin,
The surface contraction waves of Xenopus eggs reflect the metachronous cell-cycle state of the cytoplasm.
1997,
Pubmed
,
Xenbase
Rankin,
Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates.
2005,
Pubmed
,
Xenbase
Rolef Ben-Shahar,
Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.
2008,
Pubmed
Rowland,
Building sister chromatid cohesion: smc3 acetylation counteracts an antiestablishment activity.
2009,
Pubmed
Sarkaria,
Inhibition of ATM and ATR kinase activities by the radiosensitizing agent, caffeine.
1999,
Pubmed
Skibbens,
Ctf7p is essential for sister chromatid cohesion and links mitotic chromosome structure to the DNA replication machinery.
1999,
Pubmed
Sumara,
Characterization of vertebrate cohesin complexes and their regulation in prophase.
2000,
Pubmed
,
Xenbase
Sutani,
Budding yeast Wpl1(Rad61)-Pds5 complex counteracts sister chromatid cohesion-establishing reaction.
2009,
Pubmed
Takagi,
Cloning of Xenopus orthologs of Ctf7/Eco1 acetyltransferase and initial characterization of XEco2.
2008,
Pubmed
,
Xenbase
Takahashi,
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
2004,
Pubmed
,
Xenbase
Terret,
Cohesin acetylation speeds the replication fork.
2009,
Pubmed
Tutter,
Chromosomal DNA replication in a soluble cell-free system derived from Xenopus eggs.
2006,
Pubmed
,
Xenbase
Tóth,
Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication.
1999,
Pubmed
Unal,
DNA double-strand breaks trigger genome-wide sister-chromatid cohesion through Eco1 (Ctf7).
2007,
Pubmed
Unal,
A molecular determinant for the establishment of sister chromatid cohesion.
2008,
Pubmed
Walter,
Regulation of replicon size in Xenopus egg extracts.
1997,
Pubmed
,
Xenbase
Walter,
Initiation of eukaryotic DNA replication: origin unwinding and sequential chromatin association of Cdc45, RPA, and DNA polymerase alpha.
2000,
Pubmed
,
Xenbase
Wendt,
Cohesin mediates transcriptional insulation by CCCTC-binding factor.
2008,
Pubmed
Whelan,
Cohesin acetyltransferase Esco2 is a cell viability factor and is required for cohesion in pericentric heterochromatin.
2012,
Pubmed
Wu,
A conserved motif at the C terminus of sororin is required for sister chromatid cohesion.
2011,
Pubmed
,
Xenbase
Zhang,
Acetylation of Smc3 by Eco1 is required for S phase sister chromatid cohesion in both human and yeast.
2008,
Pubmed
