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XB-ART-46592
PLoS One January 1, 2013; 8 (1): e54550.

Comparative Functional Analysis of ZFP36 Genes during Xenopus Development.

Tréguer K , Faucheux C , Veschambre P , Fédou S , Thézé N , Thiébaud P .


Abstract
ZFP36 constitutes a small family of RNA binding proteins (formerly known as the TIS11 family) that target mRNA and promote their degradation. In mammals, ZFP36 proteins are encoded by four genes and, although they show similar activities in a cellular RNA destabilization assay, there is still a limited knowledge of their mRNA targets and it is not known whether or not they have redundant functions. In the present work, we have used the Xenopus embryo, a model system allowing gain- and loss-of-function studies, to investigate, whether individual ZFP36 proteins had distinct or redundant functions. We show that overexpression of individual amphibian zfp36 proteins leads to embryos having the same defects, with alteration in somites segmentation and pronephros formation. In these embryos, members of the Notch signalling pathway such as hairy2a or esr5 mRNA are down-regulated, suggesting common targets for the different proteins. We also show that mouse Zfp36 protein overexpression gives the same phenotype, indicating an evolutionary conserved property among ZFP36 vertebrate proteins. Morpholino oligonucleotide-induced loss-of-function leads to defects in pronephros formation, reduction in tubule size and duct coiling alterations for both zfp36 and zfp36l1, indicating no functional redundancy between these two genes. Given the conservation in gene structure and function between the amphibian and mammalian proteins and the conserved mechanisms for pronephros development, our study highlights a potential and hitherto unreported role of ZFP36 gene in kidney morphogenesis.

PubMed ID: 23342169
PMC ID: PMC3546996
Article link: PLoS One

Genes referenced: aplnr bmp2 chrd.1 emb esr-5 fgf2 hes4 lhx1 myl1 myod1 notch1 odc1 pax8 rgn tbxt wnt4 wnt8a wt1 zfp36 zfp36l1 zfp36l2 zfp36l2.1 znf318
Antibodies: Somite Ab1
Morpholinos: zfp36 MO1 zfp36l1 MO1 zfp36l2 MO1


Article Images: [+] show captions
References:
Baou, 2009, Pubmed [+]


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