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Plant Physiol
2013 Mar 01;1613:1347-61. doi: 10.1104/pp.112.208934.
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Low pH, aluminum, and phosphorus coordinately regulate malate exudation through GmALMT1 to improve soybean adaptation to acid soils.
Liang C
,
Piñeros MA
,
Tian J
,
Yao Z
,
Sun L
,
Liu J
,
Shaff J
,
Coluccio A
,
Kochian LV
,
Liao H
.
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Low pH, aluminum (Al) toxicity, and low phosphorus (P) often coexist and are heterogeneously distributed in acid soils. To date, the underlying mechanisms of crop adaptation to these multiple factors on acid soils remain poorly understood. In this study, we found that P addition to acid soils could stimulate Al tolerance, especially for the P-efficient genotype HN89. Subsequent hydroponic studies demonstrated that solution pH, Al, and P levels coordinately altered soybean (Glycine max) root growth and malate exudation. Interestingly, HN89 released more malate under conditions mimicking acid soils (low pH, +P, and +Al), suggesting that root malate exudation might be critical for soybean adaptation to both Al toxicity and P deficiency on acid soils. GmALMT1, a soybean malate transporter gene, was cloned from the Al-treated root tips of HN89. Like root malate exudation, GmALMT1 expression was also pH dependent, being suppressed by low pH but enhanced by Al plus P addition in roots of HN89. Quantitative real-time PCR, transient expression of a GmALMT1-yellow fluorescent protein chimera in Arabidopsis protoplasts, and electrophysiological analysis of Xenopus laevis oocytes expressing GmALMT1 demonstrated that GmALMT1 encodes a root cell plasma membrane transporter that mediates malate efflux in an extracellular pH-dependent and Al-independent manner. Overexpression of GmALMT1 in transgenic Arabidopsis, as well as overexpression and knockdown of GmALMT1 in transgenic soybean hairy roots, indicated that GmALMT1-mediated root malate efflux does underlie soybean Al tolerance. Taken together, our results suggest that malate exudation is an important component of soybean adaptation to acid soils and is coordinately regulated by three factors, pH, Al, and P, through the regulation of GmALMT1 expression and GmALMT1 function.
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994, Pubmed,
Xenbase
Ackerman,
Hypotonicity activates a native chloride current in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Davies,
Control of and by pH.
1973,
Pubmed
Degenhardt,
Aluminum resistance in the Arabidopsis mutant alr-104 is caused by an aluminum-induced increase in rhizosphere pH.
1998,
Pubmed
Delhaize,
Aluminum Tolerance in Wheat (Triticum aestivum L.) (II. Aluminum-Stimulated Excretion of Malic Acid from Root Apices).
1993,
Pubmed
Delhaize,
Transgenic barley (Hordeum vulgare L.) expressing the wheat aluminium resistance gene (TaALMT1) shows enhanced phosphorus nutrition and grain production when grown on an acid soil.
2009,
Pubmed
Delhaize,
Aluminum Tolerance in Wheat (Triticum aestivum L.) (I. Uptake and Distribution of Aluminum in Root Apices).
1993,
Pubmed
Gruber,
HvALMT1 from barley is involved in the transport of organic anions.
2010,
Pubmed
,
Xenbase
Hoekenga,
AtALMT1, which encodes a malate transporter, is identified as one of several genes critical for aluminum tolerance in Arabidopsis.
2006,
Pubmed
,
Xenbase
Huang,
Knockout of a bacterial-type ATP-binding cassette transporter gene, AtSTAR1, results in increased aluminum sensitivity in Arabidopsis.
2010,
Pubmed
Huang,
A bacterial-type ABC transporter is involved in aluminum tolerance in rice.
2009,
Pubmed
,
Xenbase
Iuchi,
Zinc finger protein STOP1 is critical for proton tolerance in Arabidopsis and coregulates a key gene in aluminum tolerance.
2007,
Pubmed
Jemo,
Aluminum resistance of cowpea as affected by phosphorus-deficiency stress.
2007,
Pubmed
Kereszt,
Agrobacterium rhizogenes-mediated transformation of soybean to study root biology.
2007,
Pubmed
Kinraide,
Three mechanisms for the calcium alleviation of mineral toxicities.
1998,
Pubmed
Kobayashi,
Characterization of AtALMT1 expression in aluminum-inducible malate release and its role for rhizotoxic stress tolerance in Arabidopsis.
2007,
Pubmed
Kochian,
How do crop plants tolerate acid soils? Mechanisms of aluminum tolerance and phosphorous efficiency.
2004,
Pubmed
Kovermann,
The Arabidopsis vacuolar malate channel is a member of the ALMT family.
2007,
Pubmed
,
Xenbase
Koyama,
Brief exposure to low-pH stress causes irreversible damage to the growing root in Arabidopsis thaliana: pectin-Ca interaction may play an important role in proton rhizotoxicity.
2001,
Pubmed
Larsen,
Aluminum-resistant Arabidopsis mutants that exhibit altered patterns of aluminum accumulation and organic acid release from roots.
1998,
Pubmed
Liang,
Biochemical and molecular characterization of PvPAP3, a novel purple acid phosphatase isolated from common bean enhancing extracellular ATP utilization.
2010,
Pubmed
Liao,
Phosphorus and aluminum interactions in soybean in relation to aluminum tolerance. Exudation of specific organic acids from different regions of the intact root system.
2006,
Pubmed
Ligaba,
The role of phosphorus in aluminium-induced citrate and malate exudation from rape (Brassica napus).
2004,
Pubmed
Ligaba,
The BnALMT1 and BnALMT2 genes from rape encode aluminum-activated malate transporters that enhance the aluminum resistance of plant cells.
2006,
Pubmed
,
Xenbase
Ligaba,
Maize ZmALMT2 is a root anion transporter that mediates constitutive root malate efflux.
2012,
Pubmed
,
Xenbase
Lipton,
Citrate, Malate, and Succinate Concentration in Exudates from P-Sufficient and P-Stressed Medicago sativa L. Seedlings.
1987,
Pubmed
Liu,
Aluminum-activated citrate and malate transporters from the MATE and ALMT families function independently to confer Arabidopsis aluminum tolerance.
2009,
Pubmed
Lü,
Improved phosphorus acquisition by tobacco through transgenic expression of mitochondrial malate dehydrogenase from Penicillium oxalicum.
2012,
Pubmed
Ma,
Role of organic acids in detoxification of aluminum in higher plants.
2000,
Pubmed
Ma,
Aluminum tolerance genes on the short arm of chromosome 3R are linked to organic acid release in triticale.
2000,
Pubmed
Maron,
Two functionally distinct members of the MATE (multi-drug and toxic compound extrusion) family of transporters potentially underlie two major aluminum tolerance QTLs in maize.
2010,
Pubmed
,
Xenbase
Martínez-Camacho,
A pH-stating mechanism in isolated wheat (Triticum aestivum) aleurone layers involves malic acid transport.
2004,
Pubmed
Meriga,
Aluminium-induced production of oxygen radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa).
2004,
Pubmed
Meyer,
AtALMT12 represents an R-type anion channel required for stomatal movement in Arabidopsis guard cells.
2010,
Pubmed
,
Xenbase
Pellet,
Multiple Aluminum-Resistance Mechanisms in Wheat (Roles of Root Apical Phosphate and Malate Exudation).
1996,
Pubmed
Piñeros,
Novel properties of the wheat aluminum tolerance organic acid transporter (TaALMT1) revealed by electrophysiological characterization in Xenopus Oocytes: functional and structural implications.
2008,
Pubmed
,
Xenbase
Piñeros,
Not all ALMT1-type transporters mediate aluminum-activated organic acid responses: the case of ZmALMT1 - an anion-selective transporter.
2008,
Pubmed
,
Xenbase
Ramos-Díaz,
Aluminum inhibits phosphatidic acid formation by blocking the phospholipase C pathway.
2007,
Pubmed
Rudrappa,
Root-secreted malic acid recruits beneficial soil bacteria.
2008,
Pubmed
Ryan,
FUNCTION AND MECHANISM OF ORGANIC ANION EXUDATION FROM PLANT ROOTS.
2001,
Pubmed
Ryan,
Aluminum Toxicity in Roots : Correlation among Ionic Currents, Ion Fluxes, and Root Elongation in Aluminum-Sensitive and Aluminum-Tolerant Wheat Cultivars.
1992,
Pubmed
Sas,
Excess cation uptake, and extrusion of protons and organic acid anions by Lupinus albus under phosphorus deficiency.
2001,
Pubmed
Sasaki,
A wheat gene encoding an aluminum-activated malate transporter.
2004,
Pubmed
,
Xenbase
Sawaki,
STOP1 regulates multiple genes that protect arabidopsis from proton and aluminum toxicities.
2009,
Pubmed
Sivaguru,
Impacts of aluminum on the cytoskeleton of the maize root apex. short-term effects on the distal part of the transition zone.
1999,
Pubmed
Sivaguru,
Aluminum-induced 1-->3-beta-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata. A new mechanism of aluminum toxicity in plants.
2000,
Pubmed
Sun,
Phosphorus enhances Al resistance in Al-resistant Lespedeza bicolor but not in Al-sensitive L. cuneata under relatively high Al stress.
2008,
Pubmed
Tokimasa,
Effects of barium, lanthanum and gadolinium on endogenous chloride and potassium currents in Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Tzfira,
pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants.
2005,
Pubmed
Walker,
Root exudation and rhizosphere biology.
2003,
Pubmed
Yamaji,
A zinc finger transcription factor ART1 regulates multiple genes implicated in aluminum tolerance in rice.
2009,
Pubmed
Yan,
Effect of Low Root Medium pH on Net Proton Release, Root Respiration, and Root Growth of Corn (Zea mays L.) and Broad Bean (Vicia faba L.).
1992,
Pubmed
Yang,
Aluminium resistance requires resistance to acid stress: a case study with spinach that exudes oxalate rapidly when exposed to Al stress.
2005,
Pubmed
Yoo,
Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis.
2007,
Pubmed
Zheng,
Immobilization of aluminum with phosphorus in roots is associated with high aluminum resistance in buckwheat.
2005,
Pubmed
