Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-46753
Elife. February 26, 2013; 2 e00348.

Epigenetic conservation at gene regulatory elements revealed by non-methylated DNA profiling in seven vertebrates.

Long HK , Sims D , Heger A , Blackledge NP , Kutter C , Wright ML , Grützner F , Odom DT , Patient R , Ponting CP , Klose RJ .


Abstract
Two-thirds of gene promoters in mammals are associated with regions of non-methylated DNA, called CpG islands (CGIs), which counteract the repressive effects of DNA methylation on chromatin. In cold-blooded vertebrates, computational CGI predictions often reside away from gene promoters, suggesting a major divergence in gene promoter architecture across vertebrates. By experimentally identifying non-methylated DNA in the genomes of seven diverse vertebrates, we instead reveal that non-methylated islands (NMIs) of DNA are a central feature of vertebrate gene promoters. Furthermore, NMIs are present at orthologous genes across vast evolutionary distances, revealing a surprising level of conservation in this epigenetic feature. By profiling NMIs in different tissues and developmental stages we uncover a unifying set of features that are central to the function of NMIs in vertebrates. Together these findings demonstrate an ancient logic for NMI usage at gene promoters and reveal an unprecedented level of epigenetic conservation across vertebrate evolution. DOI:http://dx.doi.org/10.7554/eLife.00348.001.

PubMed ID: 23467541
PMC ID: PMC3583005
Article link: Elife.
Grant support: A15603 Cancer Research UK, MC_U137981013 Medical Research Council , 15603 Cancer Research UK, 202218 European Research Council, MC_UU_12009/8 Medical Research Council , MC_UU_12009/8 Medical Research Council , MC_U137761446 Medical Research Council , WT0834922 Wellcome Trust , 202218 European Research Council, MC_U137981013 Medical Research Council , G1000902 Medical Research Council , 15603 Cancer Research UK, A15603 Cancer Research UK, 090018/Z/09/Z Wellcome Trust , WT0834922 Wellcome Trust , G1000902 Medical Research Council , CRUK_15603 Cancer Research UK, MRC_MC_U137981013 Medical Research Council , CRUK_A15603 Cancer Research UK, MRC_MC_U137761446 Medical Research Council , MRC_MC_UU_12009/8 Medical Research Council , ERC_202218 European Research Council, 090018/Z/09/Z Wellcome Trust , MC_UU_12009/8 Medical Research Council , MC_U137761446 Medical Research Council , WT0834922 Wellcome Trust , 202218 European Research Council, MC_U137981013 Medical Research Council , G1000902 Medical Research Council , 15603 Cancer Research UK, A15603 Cancer Research UK, 090018/Z/09/Z Wellcome Trust

Genes referenced: act3 aptx dnaja1 ezh2 gnao1 gsx1 nkx2-2 nmi rnf2 smu1 sp9 suz12


References:
Aday, 2011, Pubmed[+]


Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [4.6.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556