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Localization of epitopes and functional effects of two novel monoclonal antibodies against skeletal muscle myosin.
Dan-Goor M
,
Silberstein L
,
Kessel M
,
Muhlrad A
.
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Two skeletal myosin monoclonal antibodies, raised against human skeletal myosin, were used to study the correlation between function, primary and tertiary structure of S-1 prepared from rabbit skeletal myosin. The heavy chain of S-1 is cleaved into three fragments by trypsin--27 kDa, 50 kDa and 20 kDa--aligned in this order from the N-terminus. The epitope of the first antibody was assigned to the N-terminal 1-23 amino acid stretch of S-1, since it reacted with the 27 kDa N-terminal tryptic fragment of S-1 but not with a derivative of the 27 kDa fragment, which lacks the above amino acid stretch. The epitope of the second antibody was assigned to the 3 kDa N-terminal region of the central 50 kDa domain of S-1. This assignment was based on proteolytic and photochemical cleavage of S-1 and on the labelling of its N-terminus by a specific antibody. The antibodies were visualized binding to the myosin head on electron micrographs of rotary-shadowed complexes of antibodies with myosin. Measurements on the micrographs indicated that the distances between the head-tail junction of myosin and the 'anti-27 K' and 'anti-50 K' epitopes are 14 nm and 17 nm, respectively. Both antibodies have a high affinity to S-1. The affinity of the 'anti-50 K' to S-1 decreased upon actin binding, while that of the 'anti-27 K' was not affected by binding of S-1 to F-actin. The 'anti-50 K' antibody inhibited the K+ (EDTA) and the actin-activated ATPase activity of S-1, while the 'anti-27 K' had no effect. The results indicate that either the epitope of the 'anti-50 K' is near to the actin or to the ATP-binding sites of S-1, or that there is communication, expressed as propagated conformational changes, between these sites and the epitope.
Applegate,
Protease-sensitive regions in myosin subfragment 1.
1983, Pubmed
Applegate,
Protease-sensitive regions in myosin subfragment 1.
1983,
Pubmed
Botts,
On the origin and transmission of force in actomyosin subfragment 1.
1989,
Pubmed
Botts,
On the mechanism of energy transduction in myosin subfragment 1.
1984,
Pubmed
Bruck,
Purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel Blue chromatography.
1986,
Pubmed
Burke,
Substructure of skeletal myosin subfragment 1. Preferential destabilization of a domain by methanol and its effect on catalytic activity.
1986,
Pubmed
Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed
Bálint,
Location of SH-1 and SH-2 in the heavy chain segment of heavy meromyosin.
1978,
Pubmed
Chaussepied,
Abolition of ATPase activities of skeletal myosin subfragment 1 by a new selective proteolytic cleavage within the 50-kilodalton heavy chain segment.
1986,
Pubmed
Chaussepied,
Properties of the alkali light-chain-20-kilodalton fragment complex from skeletal myosin heads.
1986,
Pubmed
Degani,
Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues.
1974,
Pubmed
Deutsch,
Characterization of monoclonal antibodies to human platelet myosin that recognize highly conserved epitopes within the 50 kDa fragment of myosin subfragment-1.
1990,
Pubmed
Flicker,
Site-specific inhibition of myosin-mediated motility in vitro by monoclonal antibodies.
1985,
Pubmed
Jameson,
The antigenic index: a novel algorithm for predicting antigenic determinants.
1988,
Pubmed
Kearney,
A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
1979,
Pubmed
Lowey,
Substructure of the myosin molecule. I. Subfragments of myosin by enzymic degradation.
1969,
Pubmed
Mahmood,
Photochemical probes of the active site of myosin. Irradiation of trapped 3'-O-(4-benzoyl)benzoyladenosine 5'-triphosphate labels the 50-kilodalton heavy chain tryptic peptide.
1984,
Pubmed
Mocz,
Vanadate-mediated photocleavage of rabbit skeletal myosin.
1989,
Pubmed
Mornet,
The limited tryptic cleavage of chymotryptic S-1: an approach to the characterization of the actin site in myosin heads.
1979,
Pubmed
Mornet,
Molecular movements promoted by metal nucleotides in the heavy-chain regions of myosin heads from skeletal muscle.
1985,
Pubmed
Mornet,
Proteolysis and the domain organization of myosin subfragment 1.
1984,
Pubmed
Mornet,
Structure of the actin-myosin interface.
1981,
Pubmed
Muhlrad,
Isolation and characterization of the N-terminal 23-kilodalton fragment of myosin subfragment 1.
1989,
Pubmed
Muhlrad,
Characterization of the isolated 20 kDa and 50 kDa fragments of the myosin head.
1986,
Pubmed
Muhlrad,
Isolation and partial renaturation of proteolytic fragments of the myosin head.
1984,
Pubmed
Setton,
Effect of mild heat treatment on actin and nucleotide binding of myosin subfragment 1.
1988,
Pubmed
Setton,
Effect of mild heat treatment on the ATPase activity and proteolytic sensitivity of myosin subfragment-1.
1984,
Pubmed
Silberstein,
Developmental progression of myosin gene expression in cultured muscle cells.
1986,
Pubmed
Spudich,
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
1971,
Pubmed
Sutoh,
Mapping of actin-binding sites on the heavy chain of myosin subfragment 1.
1983,
Pubmed
Sutoh,
Electron microscopic visualization of the SH1 thiol of myosin by the use of an avidin-biotin system.
1984,
Pubmed
Sutoh,
A short hydrophobic segment next to tryptophan-130 in myosin heavy chain is close to the ribose ring of ADP bound in the adenosinetriphosphatase site.
1987,
Pubmed
Sutoh,
Electron microscopic visualization of the ATPase site of myosin by photoaffinity labeling with a biotinylated photoreactive ADP analog.
1986,
Pubmed
Sutoh,
Electron microscopic visualization of the N terminus of the myosin heavy chain using a site-directed antibody.
1987,
Pubmed
Taylor,
A new model for the geometry of the binding of myosin crossbridges to muscle thin filaments.
1981,
Pubmed
Tokunaga,
Position of the amino terminus of myosin light chain 1 and light chain 2 determined by electron microscopy with monoclonal antibody.
1987,
Pubmed
Tong,
The sequence of the NH2-terminal 204-residue fragment of the heavy chain of rabbit skeletal muscle myosin.
1983,
Pubmed
Tonomura,
On the molecular weight of myosin. II.
1966,
Pubmed
Toyoshima,
Three-dimensional image analysis of the complex of thin filaments and myosin molecules from skeletal muscle. IV. Reconstitution from minimal- and high-dose images of the actin-tropomyosin-myosin subfragment-1 complex.
1985,
Pubmed
Warrick,
Myosin structure and function in cell motility.
1987,
Pubmed
Weeds,
Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin.
1975,
Pubmed
Winkelmann,
Probing myosin head structure with monoclonal antibodies.
1986,
Pubmed
Winkelmann,
Monoclonal antibodies localize changes on myosin heavy chain isozymes during avian myogenesis.
1983,
Pubmed
