Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell
2013 Mar 28;1531:166-77. doi: 10.1016/j.cell.2013.02.037.
Show Gene links
Show Anatomy links
An RNA degradation machine sculpted by Ro autoantigen and noncoding RNA.
Chen X
,
Taylor DW
,
Fowler CC
,
Galan JE
,
Wang HW
,
Wolin SL
.
???displayArticle.abstract???
Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In the only studied bacterium, Deinococcus radiodurans, the Ro ortholog Rsr functions in heat-stress-induced ribosomal RNA (rRNA) maturation and starvation-induced rRNA decay. However, the mechanism by which this conserved protein and its associated ncRNAs act has been obscure. We report that Rsr and the exoribonuclease polynucleotide phosphorylase (PNPase) form an RNA degradation machine that is scaffolded by Y RNA. Single-particle electron microscopy, followed by docking of atomic models into the reconstruction, suggests that Rsr channels single-stranded RNA into the PNPase cavity. Biochemical assays reveal that Rsr and Y RNA adapt PNPase for effective degradation of structured RNAs. A Ro ortholog and ncRNA also associate with PNPase in Salmonella Typhimurium. Our studies identify another ribonucleoprotein machine and demonstrate that ncRNA, by tethering a protein cofactor, can alter the substrate specificity of an enzyme.
Ataide,
The crystal structure of the signal recognition particle in complex with its receptor.
2011, Pubmed
Ataide,
The crystal structure of the signal recognition particle in complex with its receptor.
2011,
Pubmed
Bonneau,
The yeast exosome functions as a macromolecular cage to channel RNA substrates for degradation.
2009,
Pubmed
Bouffard,
Interaction cloning and characterization of RoBPI, a novel protein binding to human Ro ribonucleoproteins.
2000,
Pubmed
Chen,
Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation.
2000,
Pubmed
Chen,
The Ro autoantigen binds misfolded U2 small nuclear RNAs and assists mammalian cell survival after UV irradiation.
2003,
Pubmed
,
Xenbase
Chen,
An ortholog of the Ro autoantigen functions in 23S rRNA maturation in D. radiodurans.
2007,
Pubmed
Cheng,
An important role for RNase R in mRNA decay.
2005,
Pubmed
Evguenieva-Hackenberg,
Rrp4 and Csl4 are needed for efficient degradation but not for polyadenylation of synthetic and natural RNA by the archaeal exosome.
2008,
Pubmed
Evguenieva-Hackenberg,
The archaeal exosome.
2011,
Pubmed
Fabini,
The heterogeneous nuclear ribonucleoproteins I and K interact with a subset of the ro ribonucleoprotein-associated Y RNAs in vitro and in vivo.
2001,
Pubmed
Fouraux,
Nucleolin associates with a subset of the human Ro ribonucleoprotein complexes.
2002,
Pubmed
Fuchs,
Structural and biochemical basis for misfolded RNA recognition by the Ro autoantigen.
2006,
Pubmed
,
Xenbase
Geiss,
Ring around the Ro-sie: RNA-mediated alterations of PNPase activity.
2013,
Pubmed
Genschik,
Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon.
1998,
Pubmed
Green,
Binding of the 60-kDa Ro autoantigen to Y RNAs: evidence for recognition in the major groove of a conserved helix.
1998,
Pubmed
,
Xenbase
Górna,
From conformational chaos to robust regulation: the structure and function of the multi-enzyme RNA degradosome.
2012,
Pubmed
Hannon,
The expanding universe of noncoding RNAs.
2006,
Pubmed
Hogg,
Human Y5 RNA specializes a Ro ribonucleoprotein for 5S ribosomal RNA quality control.
2007,
Pubmed
Kastner,
GraFix: sample preparation for single-particle electron cryomicroscopy.
2008,
Pubmed
Labbé,
The levels of the RoRNP-associated Y RNA are dependent upon the presence of ROP-1, the Caenorhabditis elegans Ro60 protein.
1999,
Pubmed
Lerner,
Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus.
1981,
Pubmed
Lorentzen,
Structure of the active subunit of the yeast exosome core, Rrp44: diverse modes of substrate recruitment in the RNase II nuclease family.
2008,
Pubmed
Lunde,
RNA-binding proteins: modular design for efficient function.
2007,
Pubmed
Mohanty,
Bacterial/archaeal/organellar polyadenylation.
2011,
Pubmed
O'Brien,
A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.
1994,
Pubmed
,
Xenbase
Perreault,
Ro-associated Y RNAs in metazoans: evolution and diversification.
2007,
Pubmed
Ramesh,
Crystal structure of Rsr, an ortholog of the antigenic Ro protein, links conformational flexibility to RNA binding activity.
2007,
Pubmed
Schmidt,
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
2007,
Pubmed
Shi,
A misfolded form of 5S rRNA is complexed with the Ro and La autoantigens.
1996,
Pubmed
,
Xenbase
Sim,
The subcellular distribution of an RNA quality control protein, the Ro autoantigen, is regulated by noncoding Y RNA binding.
2009,
Pubmed
Sim,
Emerging roles for the Ro 60-kDa autoantigen in noncoding RNA metabolism.
2011,
Pubmed
,
Xenbase
Sim,
The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA.
2012,
Pubmed
Sohlberg,
The Streptomyces coelicolor polynucleotide phosphorylase homologue, and not the putative poly(A) polymerase, can polyadenylate RNA.
2003,
Pubmed
Spickler,
Action of RNase II and polynucleotide phosphorylase against RNAs containing stem-loops of defined structure.
2000,
Pubmed
Stein,
Structural insights into RNA quality control: the Ro autoantigen binds misfolded RNAs via its central cavity.
2005,
Pubmed
,
Xenbase
Suloway,
Automated molecular microscopy: the new Leginon system.
2005,
Pubmed
Symmons,
A duplicated fold is the structural basis for polynucleotide phosphorylase catalytic activity, processivity, and regulation.
2000,
Pubmed
Tanaka,
RtcB is the RNA ligase component of an Escherichia coli RNA repair operon.
2011,
Pubmed
Teunissen,
Conserved features of Y RNAs: a comparison of experimentally derived secondary structures.
2000,
Pubmed
Wurtmann,
A role for a bacterial ortholog of the Ro autoantigen in starvation-induced rRNA degradation.
2010,
Pubmed
Yoshioka,
Automation of random conical tilt and orthogonal tilt data collection using feature-based correlation.
2007,
Pubmed