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J Physiol Biochem
2014 Mar 01;701:185-91. doi: 10.1007/s13105-013-0292-1.
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Mas-related G protein-coupled receptor D is coupled to endogenous calcium-activated chloride channel in Xenopus oocytes.
Zhuo RG
,
Ma XY
,
Zhou PL
,
Liu XY
,
Zhang K
,
Wei XL
,
Yan HT
,
Xu JP
,
Zheng JQ
.
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Mas-related G protein-coupled receptor D (MrgD) is expressed almost exclusively in nociceptive primary sensory neurons and the neurons located in stratum granulosum of skin. More and more evidence suggest that MrgD plays an important role in pain sensation and/or transduction. Recent studies have demonstrated that the receptor is also involved in itch sensation in both mouse and human. In the present study, we identified a robust inward current in MrgD-expressing Xenopus oocytes by using β-alanine, a putative ligand of MrgD. The currents were sensitive to inhibitor of Ca(2+)-activated chloride channels (CaCCs) and intracellular Ca(2+) chelator, suggesting they were produced by endogenous CaCCs. Furthermore, it was demonstrated that upon the application of phospholipase C (PLC) inhibitor, or antisense oligonucleotides of inositol trisphosphate receptor (IP3R), the β-alanine-induced currents were dramatically depressed. However, protein kinase C inhibitor did not display any visible effect on CaCC currents. In summary, our data suggest that the activation of MrgD promotes the open of endogenous CaCCs via G(q)-PLC-IP3-Ca(2+) pathway. The current findings reveal the functional coupling between MrgD and CaCCs in Xenopus oocytes and also provide a facile model to assay the activity of MrgD.
Acuña-Castillo,
Differences in potency and efficacy of a series of phenylisopropylamine/phenylethylamine pairs at 5-HT(2A) and 5-HT(2C) receptors.
2002, Pubmed,
Xenbase
Acuña-Castillo,
Differences in potency and efficacy of a series of phenylisopropylamine/phenylethylamine pairs at 5-HT(2A) and 5-HT(2C) receptors.
2002,
Pubmed
,
Xenbase
André,
Axotomy-induced expression of calcium-activated chloride current in subpopulations of mouse dorsal root ganglion neurons.
2003,
Pubmed
Arellano,
Agonist-activated Ca2+ influx and Ca2+ -dependent Cl- channels in Xenopus ovarian follicular cells: functional heterogeneity within the cell monolayer.
2012,
Pubmed
,
Xenbase
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
Cavanaugh,
Distinct subsets of unmyelinated primary sensory fibers mediate behavioral responses to noxious thermal and mechanical stimuli.
2009,
Pubmed
Cohen,
Pain-associated signals, acidosis and lysophosphatidic acid, modulate the neuronal K(2P)2.1 channel.
2009,
Pubmed
,
Xenbase
Crozier,
MrgD activation inhibits KCNQ/M-currents and contributes to enhanced neuronal excitability.
2007,
Pubmed
Dussor,
Cutaneous sensory neurons expressing the Mrgprd receptor sense extracellular ATP and are putative nociceptors.
2008,
Pubmed
Eggermont,
Calcium-activated chloride channels: (un)known, (un)loved?
2004,
Pubmed
Frings,
Primary processes in sensory cells: current advances.
2009,
Pubmed
Frings,
Primary processes in sensory cells: current advances.
2012,
Pubmed
Frings,
Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species.
2000,
Pubmed
Gomez-Hernandez,
Calcium dependence and distribution of calcium-activated chloride channels in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Grønlien,
Importance of M2-M3 loop in governing properties of genistein at the α7 nicotinic acetylcholine receptor inferred from α7/5-HT3A chimera.
2010,
Pubmed
,
Xenbase
Hartzell,
Calcium-activated chloride channels.
2005,
Pubmed
Huang,
Calcium-activated chloride channels (CaCCs) regulate action potential and synaptic response in hippocampal neurons.
2012,
Pubmed
Ivanenko,
A caffeine- and ryanodine-sensitive Ca2+ store in avian sensory neurons.
1993,
Pubmed
Kawano,
Activation mechanism of Ca(2+)-sensitive transient outward current in rabbit ventricular myocytes.
1995,
Pubmed
Kenyon,
Temperature dependencies of Ca2+ current, Ca(2+)-activated Cl- current and Ca2+ transients in sensory neurones.
1998,
Pubmed
Kume,
The Xenopus IP3 receptor: structure, function, and localization in oocytes and eggs.
1993,
Pubmed
,
Xenbase
Large,
Characteristics and physiological role of the Ca(2+)-activated Cl- conductance in smooth muscle.
1996,
Pubmed
Liu,
Mechanisms of itch evoked by β-alanine.
2012,
Pubmed
Liu,
The acute nociceptive signals induced by bradykinin in rat sensory neurons are mediated by inhibition of M-type K+ channels and activation of Ca2+-activated Cl- channels.
2010,
Pubmed
Lupu-Meiri,
Hemispheric asymmetry of rapid chloride responses to inositol trisphosphate and calcium in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Machaca,
Adenophostin A and inositol 1,4,5-trisphosphate differentially activate Cl- currents in Xenopus oocytes because of disparate Ca2+ release kinetics.
1999,
Pubmed
,
Xenbase
Miledi,
A calcium-dependent transient outward current in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Oh,
Development of selective blockers for Ca²(+)-activated Cl channel using Xenopus laevis oocytes with an improved drug screening strategy.
2008,
Pubmed
,
Xenbase
Rau,
Mrgprd enhances excitability in specific populations of cutaneous murine polymodal nociceptors.
2009,
Pubmed
Segal-Hayoun,
Molecular mechanisms underlying membrane-potential-mediated regulation of neuronal K2P2.1 channels.
2010,
Pubmed
,
Xenbase
Shinohara,
Identification of a G protein-coupled receptor specifically responsive to beta-alanine.
2004,
Pubmed
Takahashi,
Rat brain serotonin receptors in Xenopus oocytes are coupled by intracellular calcium to endogenous channels.
1987,
Pubmed
,
Xenbase
Tohda,
Serotonin 2C receptor (5-HT2CR) mRNA editing-induced down-regulation of 5-HT2CR function in Xenopus oocytes: the significance of site C editing.
2010,
Pubmed
,
Xenbase
Ward,
The spatial relationship between Ca2+ channels and Ca2+-activated channels and the function of Ca2+-buffering in avian sensory neurons.
2000,
Pubmed
Zhang,
Cloning and expression of MRG receptors in macaque, mouse, and human.
2005,
Pubmed
Zylka,
Topographically distinct epidermal nociceptive circuits revealed by axonal tracers targeted to Mrgprd.
2005,
Pubmed
Zylka,
Atypical expansion in mice of the sensory neuron-specific Mrg G protein-coupled receptor family.
2003,
Pubmed
