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XB-ART-47534
J Biol Chem. May 6, 2011; 286 (18): 15625-9.

Structure and histone binding properties of the Vps75-Rtt109 chaperone-lysine acetyltransferase complex.

Su D , Hu Q , Zhou H , Thompson JR , Xu RM , Zhang Z , Mer G .


Abstract
The histone chaperone Vps75 presents the remarkable property of stimulating the Rtt109-dependent acetylation of several histone H3 lysine residues within (H3-H4)(2) tetramers. To investigate this activation mechanism, we determined x-ray structures of full-length Vps75 in complex with full-length Rtt109 in two crystal forms. Both structures show similar asymmetric assemblies of a Vps75 dimer bound to an Rtt109 monomer. In the Vps75-Rtt109 complexes, the catalytic site of Rtt109 is confined to an enclosed space that can accommodate the N-terminal tail of histone H3 in (H3-H4)(2). Investigation of Vps75-Rtt109-(H3-H4)(2) and Vps75-(H3-H4)(2) complexes by NMR spectroscopy-probed hydrogen/deuterium exchange suggests that Vps75 guides histone H3 in the catalytic enclosure. These findings clarify the basis for the enhanced acetylation of histone H3 tail residues by Vps75-Rtt109.

PubMed ID: 21454705
PMC ID: PMC3091171
Article link: J Biol Chem.
Grant support: CA132878 NCI NIH HHS , CA132878 NCI NIH HHS , CA132878 NCI NIH HHS , CA132878 NCI NIH HHS , CA132878 NCI NIH HHS , CA132878 NCI NIH HHS , GM81838 NIGMS NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-03 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-04 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS , R01 CA132878-05 NCI NIH HHS

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