Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Chick myotendinous antigen. I. A monoclonal antibody as a marker for tendon and muscle morphogenesis.
Chiquet M
,
Fambrough DM
.
???displayArticle.abstract??? Extracellular matrix components are likely to be involved in the interaction of muscle with nonmuscle cells during morphogenesis and in adult skeletal muscle. With the aim of identifying relevant molecules, we generated monoclonal antibodies that react with the endomysium, i.e., the extracellular matrix on the surface of single muscle fibers. Antibody M1, which is described here, specifically labeled the endomysium of chick anterior latissimus dorsi muscle (but neither the perimysium nor, with the exception of blood vessels and perineurium, the epimysium ). Endomysium labeling was restricted to proximal and distal portions of muscle fibers near their insertion points to tendon, but absent from medial regions of the muscle. Myotendinous junctions and tendon fascicles were intensely labeled by M1 antibody. In chick embryos, " myotendinous antigen" (as we tentatively call the epitope recognized by M1 antibody) appeared first in the perichondrium of vertebrae and limbcartilage elements, from where it gradually extended to the premuscle masses. Around day 6, tendonprimordia were clearly labeled. The other structures labeled by M1 antibody in chick embryos were developing smooth muscle tissues, especially aorta, gizzard, and lung buds. In general, tissues labeled with M1 antibody appeared to be a subset of the ones accumulating fibronectin. In cell cultures, M1 antibody binds to fuzzy, fibrillar material on the substrate and cell surfaces of living fibroblast and myogenic cells, which confirms an extracellular location of the antigenic site. The appearance of myotendinous antigen during limb morphogenesis and its distribution in adult muscle and tendon are compatible with the idea that it might be involved in attaching muscle fibers to tendon fascicles. Its biochemical characterization is described in the accompanying paper ( Chiquet , M., and D. Fambrough , 1984, J. Cell Biol. 98:1937-1946).
Anderson,
Aggregates of acetylcholine receptors are associated with plaques of a basal lamina heparan sulfate proteoglycan on the surface of skeletal muscle fibers.
1983, Pubmed,
Xenbase
Anderson,
Aggregates of acetylcholine receptors are associated with plaques of a basal lamina heparan sulfate proteoglycan on the surface of skeletal muscle fibers.
1983,
Pubmed
,
Xenbase
Bailey,
Identification and change of collagen types in differentiating myoblasts and developing chick muscle.
1979,
Pubmed
Chiquet,
Muscle morphogenesis: Evidence for an organizing function of exogenous fibronectin.
1981,
Pubmed
Chiquet,
Cellular origin of extracellular matrix components during muscle morphogenesis revealed by monoclonal antibodies.
1982,
Pubmed
Chiquet,
Chick myotendinous antigen. II. A novel extracellular glycoprotein complex consisting of large disulfide-linked subunits.
1984,
Pubmed
Christ,
[Origin of wing musculature. Experimental studies on quail and chick embryos].
1974,
Pubmed
Dessau,
Synthesis and extracellular deposition of fibronectin in chondrocyte cultures. Response to the removal of extracellular cartilage matrix.
1978,
Pubmed
Ehrismann,
Mode of action of fibronectin in promoting chicken myoblast attachment. Mr = 60,000 gelatin-binding fragment binds native fibronectin.
1981,
Pubmed
GREENWOOD,
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
1963,
Pubmed
Gardner,
Fibronectin expression during myogenesis.
1983,
Pubmed
Greenlee,
The fine structure of elastic fibers.
1966,
Pubmed
Hynes,
Fibronectins: multifunctional modular glycoproteins.
1982,
Pubmed
Kennett,
Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells.
1978,
Pubmed
Kieny,
Autonomy of tendon development in the embryonic chick wing.
1979,
Pubmed
Krieg,
Type III procollagen is the major collageneous component produced by a continuous rhabdomyosarcoma cell line.
1979,
Pubmed
Köhler,
Cellular and molecular restrictions of the lymphocyte fusion.
1978,
Pubmed
Kühl,
Synthesis of type IV collagen and laminin in cultures of skeletal muscle cells and their assembly on the surface of myotubes.
1982,
Pubmed
Leung,
Cyclic stretching stimulates synthesis of matrix components by arterial smooth muscle cells in vitro.
1976,
Pubmed
Linder,
Distribution of fibroblast surface antigen in the developing chick embryo.
1975,
Pubmed
Linsenmayer,
Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunocytochemical localization in embryonic cornea and other organs.
1983,
Pubmed
McMahan,
Cholinesterase is associated with the basal lamina at the neuromuscular junction.
1978,
Pubmed
Rucker,
Arterial elastin synthesis in the growing chick.
1977,
Pubmed
Sanes,
Laminin, fibronectin, and collagen in synaptic and extrasynaptic portions of muscle fiber basement membrane.
1982,
Pubmed
Shellswell,
Has collagen a role in muscle pattern formation in the developing chick wing? 1. An immunofluorescence study.
1980,
Pubmed
Trelstad,
Tendon collagen fibrillogenesis: intracellular subassemblies and cell surface changes associated with fibril growth.
1979,
Pubmed
Trotter,
Structure and function of the murine muscle-tendon junction.
1981,
Pubmed
Turner,
Creatine kinase and aldolase isoenzyme transitions in cultures of chick skeletal muscle cells.
1974,
Pubmed
Turner,
Guidance of myogenic cell migration by oriented deposits of fibronectin.
1983,
Pubmed
Wakshull,
Characterization of a plasma membrane glycoprotein common to myoblasts, skeletal muscle satellite cells, and glia.
1983,
Pubmed
von der Mark,
Study of differential collagen synthesis during development of the chick embryo by immunofluroescence. II. Localization of type I and type II collagen during long bone development.
1976,
Pubmed
von der Mark,
Isolation and characterization of collagen A and B chains from chick embryos.
1979,
Pubmed
