XB-ART-47561ScientificWorldJournal January 1, 2012; 2012 369802.
Assessment of tools for marker-assisted selection in a marine commercial species: significant association between MSTN-1 gene polymorphism and growth traits.
Growth is a priority trait from the point of view of genetic improvement. Molecular markers linked to quantitative trait loci (QTL) have been regarded as useful for marker-assisted selection in complex traits as growth. Polymorphisms have been studied in five candidate genes influencing growth in gilthead seabream (Sparus aurata): the growth hormone (GH), insulin-like growth factor-1 (IGF-1), myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL) genes. Specimens evaluated were from a commercial broodstock comprising 131 breeders (from which 36 males and 44 females contributed to the progeny). In all samples eleven gene fragments, covering more than 13,000 bp, generated by PCR-RFLP, were analyzed; tests were made for significant associations between these markers and growth traits. ANOVA results showed a significant association between MSTN-1 gene polymorphism and growth traits. Pairwise tests revealed several RFLPs in the MSTN-1 gene with significant heterogeneity of genotypes among size groups. PRL and MSTN-1 genes presented linkage disequilibrium. The MSTN-1 gene was mapped in the centromeric region of a medium-size acrocentric chromosome pair.
PubMed ID: 22666112
PMC ID: PMC3361217
Article link: ScientificWorldJournal
Genes referenced: igf1 ins mstn.1 mstn.2 prl.1 prl.2
Article Images: [+] show captions
|Figure 1. Schematic diagram of sampling during the different life stages.|
|Figure 2. Structure of the candidate genes and oligonucleotide primers used for amplification by PCR in this study. Growth hormone (a), Insulin-Like Growth Factor I (b), Myostatin-1 (c), Prolactin (d), and Somatolactin (e) genes. I: Introns; E: Exons; Arrows indicate relative position of the primers.|
|Figure 3. Localization of the myostatin-1 probe on S. aurata chromosomes. (a) MSTN-1 probe in the centromeric region of acrocentric chromosomes. (b) Localization of MSTN-1 and putative MSTN-2 genes both in centromeric regions of two different acrocentric chromosomes.|