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XB-ART-47737
Stem Cell Reports January 1, 2013; 1 (3): 248-65.

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Weidgang CE , Russell R , Tata PR , Kühl SJ , Illing A , Müller M , Lin Q , Brunner C , Boeckers TM , Bauer K , Kartikasari AE , Guo Y , Radenz M , Bernemann C , Weiß M , Seufferlein T , Zenke M , Iacovino M , Kyba M , Schöler HR , Kühl M , Liebau S , Kleger A .


Abstract
Cell-fate decisions and pluripotency are dependent on networks of key transcriptional regulators. Recent reports demonstrated additional functions of pluripotency-associated factors during early lineage commitment. The T-box transcription factor TBX3 has been implicated in regulating embryonic stem cell self-renewal and cardiogenesis. Here, we show that TBX3 is dynamically expressed during specification of the mesendoderm lineages in differentiating embryonic stem cells (ESCs) in vitro and in developing mouse and Xenopus embryos in vivo. Forced TBX3 expression in ESCs promotes mesendoderm specification by directly activating key lineage specification factors and indirectly by enhancing paracrine Nodal/Smad2 signaling. TBX3 loss-of-function analyses in the Xenopus underline its requirement for mesendoderm lineage commitment. Moreover, we uncovered a functional redundancy between TBX3 and Tbx2 during Xenopus gastrulation. Taken together, we define further facets of TBX3 actions and map TBX3 as an upstream regulator of the mesendoderm transcriptional program during gastrulation.

PubMed ID: 24319661
PMC ID: PMC3849240
Article link: Stem Cell Reports

Genes referenced: alb bmp4 cer1 dpp4 eomes fgf2 foxa2 hhex isl1 klf5 klf9 lefty mespb myh6 myl7 nodal nodal1 pecam1 pou5f3.1 smad2 sox17a tbx2 tbx3 tbxt vegfa wnt3a


Article Images: [+] show captions
References [+] :
Arnold, Making a commitment: cell lineage allocation and axis patterning in the early mouse embryo. 2009, Pubmed


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