Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Science
2013 Nov 15;3426160:853-6. doi: 10.1126/science.1243110.
Show Gene links
Show Anatomy links
Changes in cytoplasmic volume are sufficient to drive spindle scaling.
Hazel J
,
Krutkramelis K
,
Mooney P
,
Tomschik M
,
Gerow K
,
Oakey J
,
Gatlin JC
.
???displayArticle.abstract???
The mitotic spindle must function in cell types that vary greatly in size, and its dimensions scale with the rapid, reductive cell divisions that accompany early stages of development. The mechanism responsible for this scaling is unclear, because uncoupling cell size from a developmental or cellular context has proven experimentally challenging. We combined microfluidic technology with Xenopus egg extracts to characterize spindle assembly within discrete, geometrically defined volumes of cytoplasm. Reductions in cytoplasmic volume, rather than developmental cues or changes in cell shape, were sufficient to recapitulate spindle scaling observed in Xenopus embryos. Thus, mechanisms extrinsic to the spindle, specifically a limiting pool of cytoplasmic component(s), play a major role in determining spindle size.
Bird,
RanGTP and CLASP1 cooperate to position the mitotic spindle.
2013, Pubmed
Bird,
RanGTP and CLASP1 cooperate to position the mitotic spindle.
2013,
Pubmed
Chan,
Scaling properties of cell and organelle size.
2010,
Pubmed
Courtois,
The transition from meiotic to mitotic spindle assembly is gradual during early mammalian development.
2012,
Pubmed
Decker,
Limiting amounts of centrosome material set centrosome size in C. elegans embryos.
2011,
Pubmed
Dendukuri,
Controlled synthesis of nonspherical microparticles using microfluidics.
2005,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Duffy,
Rapid Prototyping of Microfluidic Systems in Poly(dimethylsiloxane).
1998,
Pubmed
Dumont,
Spindle size: small droplets and a big step forward.
2014,
Pubmed
Dumont,
Force and length in the mitotic spindle.
2009,
Pubmed
Gatlin,
Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules.
2009,
Pubmed
,
Xenbase
Goehring,
Organelle growth control through limiting pools of cytoplasmic components.
2012,
Pubmed
Gönczy,
Spindle positioning during the asymmetric first cell division of Caenorhabditis elegans embryos.
2001,
Pubmed
Hara,
Cell-size-dependent spindle elongation in the Caenorhabditis elegans early embryo.
2009,
Pubmed
Holy,
Assembly and positioning of microtubule asters in microfabricated chambers.
1997,
Pubmed
Kiyomitsu,
Chromosome- and spindle-pole-derived signals generate an intrinsic code for spindle position and orientation.
2012,
Pubmed
Lee,
Positioning of the mitotic spindle by a cortical-microtubule capture mechanism.
2000,
Pubmed
Ludington,
Organelle size equalization by a constitutive process.
2012,
Pubmed
Maresca,
Methods for studying spindle assembly and chromosome condensation in Xenopus egg extracts.
2006,
Pubmed
,
Xenbase
Minc,
Influence of cell geometry on division-plane positioning.
2011,
Pubmed
Mitchison,
Growth, interaction, and positioning of microtubule asters in extremely large vertebrate embryo cells.
2012,
Pubmed
,
Xenbase
Montorzi,
Xenopus laevis embryo development: arrest of epidermal cell differentiation by the chelating agent 1,10-phenanthroline.
2000,
Pubmed
,
Xenbase
Newport,
A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage.
1982,
Pubmed
,
Xenbase
Sharp,
Functional coordination of three mitotic motors in Drosophila embryos.
2000,
Pubmed
Tolić-Nørrelykke,
Positioning and elongation of the fission yeast spindle by microtubule-based pushing.
2004,
Pubmed
Wilbur,
Mitotic spindle scaling during Xenopus development by kif2a and importin α.
2013,
Pubmed
,
Xenbase
Wühr,
Evidence for an upper limit to mitotic spindle length.
2008,
Pubmed
,
Xenbase
Wühr,
How does a millimeter-sized cell find its center?
2009,
Pubmed
,
Xenbase
Wühr,
A model for cleavage plane determination in early amphibian and fish embryos.
2010,
Pubmed
,
Xenbase
