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Sci Rep January 1, 2013; 3 2278.

Single blastomere expression profiling of Xenopus laevis embryos of 8 to 32-cells reveals developmental asymmetry.

Flachsova M , Sindelka R , Kubista M .

We have measured the expression of 41 maternal mRNAs in individual blastomeres collected from the 8 to 32-cell Xenopus laevis embryos to determine when and how asymmetry in the body plan is introduced. We demonstrate that the asymmetry along the animal-vegetal axis in the oocyte is transferred to the daughter cells during early cell divisions. All studied mRNAs are distributed evenly among the set of animal as well as vegetal blastomeres. We find no asymmetry in mRNA levels that might be ascribed to the dorso-ventral specification or the left-right axis formation. We hypothesize that while the animal-vegetal asymmetry is a consequence of mRNA gradients, the dorso-ventral and left-right axes specifications are induced by asymmetric distribution of other biomolecules, probably proteins.

PubMed ID: 23880666
PMC ID: PMC3721081
Article link: Sci Rep

Species referenced: Xenopus laevis
Genes referenced: acta1 axin1 bmp2 ccni cdx1 cdx2 ctnnb1 cyc1 dazl ddx25 dvl2 dvl3 eef1a1 foxh1.2 foxr1 fzd7 gdf1 gsk3b lrp6 maml1 mapk8 mark3 mos odc1 otx1 pax6 pias1 pou5f3.3 stat3.1 stat3.2 tcf7l1 trim36 tubb vegt wnt11 zp3

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References [+] :
Aanstad, Predictability of dorso-ventral asymmetry in the cleavage stage zebrafish embryo: an analysis using lithium sensitivity as a dorso-ventral marker. 2000, Pubmed