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Silent, generic and plant kairomone sensitive odorant receptors from the Southern house mosquito.
Xu P
,
Choo YM
,
Pelletier J
,
Sujimoto FR
,
Hughes DT
,
Zhu F
,
Atungulu E
,
Cornel AJ
,
Luetje CW
,
Leal WS
.
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The Southern house mosquito Culex quinquefasciatus has the largest repertoire of odorant receptors (ORs) of all mosquitoes and dipteran species whose genomes have been sequenced to date. Previously, we have identified and de-orphanized two ORs expressed in female antennae, CquiOR2 and CquiOR10, which are sensitive to oviposition attractants. In view of a new nomenclature for the Culex genome (VectorBase) we renamed these ORs as CquiOR21 (formerly CquiOR10) and CquiOR121 (CquiOR2). In addition, we selected ORs from six different phylogenetic groups for deorphanization. We cloned four of them by using cDNA from female antennae as a template. Attempts to clone CquiOR87 and CquiOR110 were unsuccessful either because they are pseudogenes or are not expressed in adult female antennae, the main olfactory tissue. By contrast, CquiOR1, CquiOR44, CquiOR73, and CquiOR161 were highly expressed in female antennae. To de-orphanize these ORs, we employed the Xenopus oocyte recording system. CquiORx-CquiOrco-expressed oocytes were challenged with a panel of 90 compounds, including known oviposition attractants, human and vertebrate host odorants, plant kairomones, and naturally occurring repellents. While CquiOR161 did not respond to any test compound in two different laboratories, CquiOR1 showed the features of a generic OR, with strong responses to 1-octen-3-ol and other ligands. CquiOR44 and CquiOR73 showed preference to plant-derived terpenoids and phenolic compounds, respectively. While fenchone was the best ligand for the former, 3,5-dimethylphenol elicited the strongest responses in the latter. The newly de-orphanized ORs may be involved in reception of plant kairomones and/or natural repellents.
Andreadis,
The contribution of Culex pipiens complex mosquitoes to transmission and persistence of West Nile virus in North America.
2012, Pubmed
Andreadis,
The contribution of Culex pipiens complex mosquitoes to transmission and persistence of West Nile virus in North America.
2012,
Pubmed
Arensburger,
Sequencing of Culex quinquefasciatus establishes a platform for mosquito comparative genomics.
2010,
Pubmed
Birkett,
The role of volatile semiochemicals in mediating host location and selection by nuisance and disease-transmitting cattle flies.
2004,
Pubmed
Carey,
Odorant reception in the malaria mosquito Anopheles gambiae.
2010,
Pubmed
Choi,
Repellent activities of essential oils and monoterpenes against Culex pipiens pallens.
2002,
Pubmed
Hughes,
Odorant receptor from the southern house mosquito narrowly tuned to the oviposition attractant skatole.
2010,
Pubmed
,
Xenbase
Kafle,
Toxicity and repellency of compounds from clove (Syzygium aromaticum) to red imported fire ants Solenopsis invicta (Hymenoptera: Formicidae).
2013,
Pubmed
Kline,
Evaluation of 1-octen-3-ol as an attractant for Coquillettidia perturbans, Mansonia spp. and Culex spp. associated with phosphate mining operations.
1990,
Pubmed
Leal,
Reverse and conventional chemical ecology approaches for the development of oviposition attractants for Culex mosquitoes.
2008,
Pubmed
Lee,
High degree of single nucleotide polymorphisms in California Culex pipiens (Diptera: Culicidae) sensu lato.
2012,
Pubmed
Logan,
Arm-in-cage testing of natural human-derived mosquito repellents.
2010,
Pubmed
Logan,
Identification of human-derived volatile chemicals that interfere with attraction of the Scottish biting midge and their potential use as repellents.
2009,
Pubmed
Mboera,
Sampling gravid Culex quinquefasciatus (Diptera: Culicidae) in Tanzania with traps baited with synthetic oviposition pheromone and grass infusions.
2000,
Pubmed
Millar,
Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass infusions.
1992,
Pubmed
Olagbemiro,
Laboratory and field responses of the mosquito, Culex quinquefasciatus, to plant-derived Culex spp. oviposition pheromone and the oviposition cue skatole.
2004,
Pubmed
Omolo,
Repellency of essential oils of some Kenyan plants against Anopheles gambiae.
2004,
Pubmed
Paluch,
Mosquito repellents: a review of chemical structure diversity and olfaction.
2010,
Pubmed
Pelletier,
An odorant receptor from the southern house mosquito Culex pipiens quinquefasciatus sensitive to oviposition attractants.
2010,
Pubmed
,
Xenbase
Pitts,
Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding.
2011,
Pubmed
Syed,
Acute olfactory response of Culex mosquitoes to a human- and bird-derived attractant.
2009,
Pubmed
Tamura,
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.
2011,
Pubmed
Wang,
Molecular basis of odor coding in the malaria vector mosquito Anopheles gambiae.
2010,
Pubmed
,
Xenbase
