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Chromatin structure and gene expression are both regulated by nucleosome assembly. How environmental factors influence histone nuclear import and the nucleosome assembly pathway, leading to changes in chromatin organization and transcription, remains unknown. Acrolein (Acr) is an α,β-unsaturated aldehyde, which is abundant in the environment, especially in cigarette smoke. It has recently been implicated as a potential major carcinogen of smoking-related lung cancer. Here we show that Acr forms adducts with histone proteins in vitro and in vivo and preferentially reacts with free histones rather than with nucleosomal histones. Cellular fractionation analyses reveal that Acr exposure specifically inhibits acetylations of N-terminal tails of cytosolic histones H3 and H4, modifications that are important for nuclear import and chromatin assembly. Notably, Acr exposure compromises the delivery of histone H3 into chromatin and increases chromatin accessibility. Moreover, changes in nucleosome occupancy at several genomic loci are correlated with transcriptional responses to Acr exposure. Our data provide new insights into mechanisms whereby environmental factors interact with the genome and influence genome function.
Adkins,
The histone chaperone Asf1p mediates global chromatin disassembly in vivo.
2004, Pubmed
Adkins,
The histone chaperone Asf1p mediates global chromatin disassembly in vivo.
2004,
Pubmed
Aldini,
Protein modification by acrolein: relevance to pathological conditions and inhibition by aldehyde sequestering agents.
2011,
Pubmed
Basu,
Proteome-wide prediction of acetylation substrates.
2009,
Pubmed
Bein,
Acrolein - a pulmonary hazard.
2011,
Pubmed
Burcham,
Toxicity of smoke extracts towards A549 lung cells: role of acrolein and suppression by carbonyl scavengers.
2010,
Pubmed
Burcham,
Intermediate filament carbonylation during acute acrolein toxicity in A549 lung cells: functional consequences, chaperone redistribution, and protection by bisulfite.
2010,
Pubmed
Burcham,
Carbonyl-scavenging drugs & protection against carbonyl stress-associated cell injury.
2008,
Pubmed
Chung,
Formation of cyclic 1,N2-propanodeoxyguanosine adducts in DNA upon reaction with acrolein or crotonaldehyde.
1984,
Pubmed
Denissenko,
Preferential formation of benzo[a]pyrene adducts at lung cancer mutational hotspots in P53.
1996,
Pubmed
Dyer,
Reconstitution of nucleosome core particles from recombinant histones and DNA.
2004,
Pubmed
Ejlassi-Lassallette,
H4 replication-dependent diacetylation and Hat1 promote S-phase chromatin assembly in vivo.
2011,
Pubmed
Ejlassi-Lassallette,
Replication-coupled chromatin assembly of newly synthesized histones: distinct functions for the histone tail domains.
2012,
Pubmed
Faroon,
Acrolein environmental levels and potential for human exposure.
2008,
Pubmed
Faroon,
Acrolein health effects.
2008,
Pubmed
Feil,
Epigenetics and the environment: emerging patterns and implications.
2012,
Pubmed
Feng,
Acrolein is a major cigarette-related lung cancer agent: Preferential binding at p53 mutational hotspots and inhibition of DNA repair.
2006,
Pubmed
Gossett,
In vivo effects of histone H3 depletion on nucleosome occupancy and position in Saccharomyces cerevisiae.
2012,
Pubmed
Groth,
Chromatin challenges during DNA replication and repair.
2007,
Pubmed
Han,
Histone H2B repression causes cell-cycle-specific arrest in yeast: effects on chromosomal segregation, replication, and transcription.
1987,
Pubmed
Hecht,
Smoking and lung cancer--a new role for an old toxicant?
2006,
Pubmed
Jin,
Nucleosome stability mediated by histone variants H3.3 and H2A.Z.
2007,
Pubmed
Jin,
Distribution of histone H3.3 in hematopoietic cell lineages.
2006,
Pubmed
Kim,
Effects of histone H4 depletion on the cell cycle and transcription of Saccharomyces cerevisiae.
1988,
Pubmed
Kouzarides,
Chromatin modifications and their function.
2007,
Pubmed
Li,
All roads lead to chromatin: Multiple pathways for histone deposition.
2012,
Pubmed
Li,
Adenosine transporter ENT4 is a direct target of EWS/WT1 translocation product and is highly expressed in desmoplastic small round cell tumor.
2008,
Pubmed
Li,
Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development.
2007,
Pubmed
Loyola,
PTMs on H3 variants before chromatin assembly potentiate their final epigenetic state.
2006,
Pubmed
Lu,
Formaldehyde-induced histone modifications in vitro.
2008,
Pubmed
Lu,
Gene regulation and DNA damage in the ageing human brain.
2004,
Pubmed
Luo,
Mechanisms of acrolein-induced myocardial dysfunction: implications for environmental and endogenous aldehyde exposure.
2007,
Pubmed
Mercado,
Cigarette smoking as a risk factor for atherosclerosis and renal disease: novel pathogenic insights.
2007,
Pubmed
Ning,
Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease.
2004,
Pubmed
Polo,
Chromatin assembly: a basic recipe with various flavours.
2006,
Pubmed
Singh,
Role of by-products of lipid oxidation in Alzheimer's disease brain: a focus on acrolein.
2010,
Pubmed
Sithu,
Exposure to acrolein by inhalation causes platelet activation.
2010,
Pubmed
Stevens,
Acrolein: sources, metabolism, and biomolecular interactions relevant to human health and disease.
2008,
Pubmed
Stevens,
Acrolein.
2011,
Pubmed
Tang,
Acrolein induced DNA damage, mutagenicity and effect on DNA repair.
2011,
Pubmed
Thompson,
Genome-wide transcriptional responses to acrolein.
2008,
Pubmed
Thompson,
Protein alkylation, transcriptional responses and cytochrome c release during acrolein toxicity in A549 cells: influence of nucleophilic culture media constituents.
2008,
Pubmed
Tirumalai,
Acrolein causes transcriptional induction of phase II genes by activation of Nrf2 in human lung type II epithelial (A549) cells.
2002,
Pubmed
Valacchi,
Inhibition of NFkappaB activation and IL-8 expression in human bronchial epithelial cells by acrolein.
2005,
Pubmed
Verreault,
Nucleosomal DNA regulates the core-histone-binding subunit of the human Hat1 acetyltransferase.
1998,
Pubmed
Wang,
Effect of CpG methylation at different sequence context on acrolein- and BPDE-DNA binding and mutagenesis.
2013,
Pubmed
Wang,
Effect of carcinogenic acrolein on DNA repair and mutagenic susceptibility.
2012,
Pubmed
Wyrick,
Chromosomal landscape of nucleosome-dependent gene expression and silencing in yeast.
1999,
Pubmed
Zhang,
Human histone acetyltransferase 1 protein preferentially acetylates H4 histone molecules in H3.1-H4 over H3.3-H4.
2012,
Pubmed
Zhou,
Effects of nickel, chromate, and arsenite on histone 3 lysine methylation.
2009,
Pubmed
Zhu,
Histone variants and chromatin assembly in plant abiotic stress responses.
2013,
Pubmed
