Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The crowded intracellular environment poses a formidable challenge to experimental and theoretical analyses of intracellular transport mechanisms. Our measurements of single-particle trajectories in cytoplasm and their random-walk interpretations elucidate two of these mechanisms: molecular diffusion in crowded environments and cytoskeletal transport along microtubules. We employed acousto-optic deflector microscopy to map out the three-dimensional trajectories of microspheres migrating in the cytosolic fraction of a cellular extract. Classical Brownian motion (BM), continuous time random walk, and fractional BM were alternatively used to represent these trajectories. The comparison of the experimental and numerical data demonstrates that cytoskeletal transport along microtubules and diffusion in the cytosolic fraction exhibit anomalous (nonFickian) behavior and posses statistically distinct signatures. Among the three random-walk models used, continuous time random walk provides the best representation of diffusion, whereas microtubular transport is accurately modeled with fractional BM.
Banks,
Anomalous diffusion of proteins due to molecular crowding.
2005, Pubmed
Banks,
Anomalous diffusion of proteins due to molecular crowding.
2005,
Pubmed
Bates,
Dynamics of DNA molecules in a membrane channel probed by active control techniques.
2003,
Pubmed
Brockmann,
The scaling laws of human travel.
2006,
Pubmed
Bronstein,
Transient anomalous diffusion of telomeres in the nucleus of mammalian cells.
2009,
Pubmed
Brunstein,
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores.
2009,
Pubmed
,
Xenbase
Bullen,
High-speed, random-access fluorescence microscopy: I. High-resolution optical recording with voltage-sensitive dyes and ion indicators.
1997,
Pubmed
Burov,
Single particle tracking in systems showing anomalous diffusion: the role of weak ergodicity breaking.
2011,
Pubmed
Caspi,
Enhanced diffusion in active intracellular transport.
2000,
Pubmed
Caspi,
Diffusion and directed motion in cellular transport.
2002,
Pubmed
Coggan,
Evidence for ectopic neurotransmission at a neuronal synapse.
2005,
Pubmed
Deng,
Ergodic properties of fractional Brownian-Langevin motion.
2009,
Pubmed
Gardner,
Rapid microtubule self-assembly kinetics.
2011,
Pubmed
Golding,
Physical nature of bacterial cytoplasm.
2006,
Pubmed
Grewe,
High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision.
2010,
Pubmed
He,
Random time-scale invariant diffusion and transport coefficients.
2008,
Pubmed
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Jeon,
In vivo anomalous diffusion and weak ergodicity breaking of lipid granules.
2011,
Pubmed
Kerssemakers,
Assembly dynamics of microtubules at molecular resolution.
2006,
Pubmed
Khan,
Spatiotemporal maps of CaMKII in dendritic spines.
2012,
Pubmed
Kirkby,
A compact Acousto-Optic Lens for 2D and 3D femtosecond based 2-photon microscopy.
2010,
Pubmed
Kolomeisky,
Molecular motors: a theorist's perspective.
2007,
Pubmed
Köpf,
Anomalous diffusion of water in biological tissues.
1996,
Pubmed
Lubelski,
Nonergodicity mimics inhomogeneity in single particle tracking.
2008,
Pubmed
Luby-Phelps,
Cytoarchitecture and physical properties of cytoplasm: volume, viscosity, diffusion, intracellular surface area.
2000,
Pubmed
Medalia,
Macromolecular architecture in eukaryotic cells visualized by cryoelectron tomography.
2002,
Pubmed
Minton,
The influence of macromolecular crowding and macromolecular confinement on biochemical reactions in physiological media.
2001,
Pubmed
Muramatsu,
Tracer diffusion of globular proteins in concentrated protein solutions.
1988,
Pubmed
Neusius,
Subdiffusion in time-averaged, confined random walks.
2009,
Pubmed
Ozarslan,
Observation of anomalous diffusion in excised tissue by characterizing the diffusion-time dependence of the MR signal.
2006,
Pubmed
Qian,
Single particle tracking. Analysis of diffusion and flow in two-dimensional systems.
1991,
Pubmed
Salomé,
Ultrafast random-access scanning in two-photon microscopy using acousto-optic deflectors.
2006,
Pubmed
Seisenberger,
Real-time single-molecule imaging of the infection pathway of an adeno-associated virus.
2001,
Pubmed
Slepchenko,
Switching of membrane organelles between cytoskeletal transport systems is determined by regulation of the microtubule-based transport.
2007,
Pubmed
Tolić-Nørrelykke,
Anomalous diffusion in living yeast cells.
2004,
Pubmed
Türkcan,
A Bayesian inference scheme to extract diffusivity and potential fields from confined single-molecule trajectories.
2012,
Pubmed
Vale,
The molecular motor toolbox for intracellular transport.
2003,
Pubmed
Verkman,
Solute and macromolecule diffusion in cellular aqueous compartments.
2002,
Pubmed
Vucinić,
A compact multiphoton 3D imaging system for recording fast neuronal activity.
2007,
Pubmed
Wang,
Forward transport of proteins in the plasma membrane of migrating cerebellar granule cells.
2012,
Pubmed
