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XB-ART-48197
J Gen Physiol. March 1, 2013; 141 (3): 275-85.

Molecular mechanism of voltage sensing in voltage-gated proton channels.

Gonzalez C , Rebolledo S , Perez ME , Larsson HP .


Abstract
Voltage-gated proton (Hv) channels play an essential role in phagocytic cells by generating a hyperpolarizing proton current that electrically compensates for the depolarizing current generated by the NADPH oxidase during the respiratory burst, thereby ensuring a sustained production of reactive oxygen species by the NADPH oxidase in phagocytes to neutralize engulfed bacteria. Despite the importance of the voltage-dependent Hv current, it is at present unclear which residues in Hv channels are responsible for the voltage activation. Here we show that individual neutralizations of three charged residues in the fourth transmembrane domain, S4, all reduce the voltage dependence of activation. In addition, we show that the middle S4 charged residue moves from a position accessible from the cytosolic solution to a position accessible from the extracellular solution, suggesting that this residue moves across most of the membrane electric field during voltage activation of Hv channels. Our results show for the first time that the charge movement of these three S4 charges accounts for almost all of the measured gating charge in Hv channels.

PubMed ID: 23401575
PMC ID: PMC3581690
Article link: J Gen Physiol.
Grant support: R01 HL095920 NHLBI NIH HHS , R01 HL095920 NHLBI NIH HHS , R01 HL095920 NHLBI NIH HHS , R01 HL095920 NHLBI NIH HHS , R01 HL095920 NHLBI NIH HHS , R01 HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS , R01-HL095920 NHLBI NIH HHS

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