Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Planta
1988 Nov 01;1762:149-58. doi: 10.1007/BF00392440.
Show Gene links
Show Anatomy links
Import of peroxisomal hydroxypyruvate reductase into glyoxysomes.
Sautter C
,
Sautter E
,
Hock B
.
???displayArticle.abstract???
A new procedure was used to purify the peroxisomal matrix enzyme hydroxypyruvate reductase (HPR) from green leaves of pumpkin (Cucurbita pepo L.) and spinach (Spinacia oleracea L.). Monospecific antibodies were prepared against this enzyme in rabbits. Immunoprecipitation of HPR from watermelon (Citrullus vulgaris Schrad.) yielded a single protein with a subunit molecular weight of 45 kDa. Immunohistochemical labeling of HPR was found exclusively in watermelon microbodies. Isolated polyadenylated mRNA from light-grown watermelon cotyledons was injected into Xenopus laevis oocytes. The heterologous in-vivo translation product of HPR exhibited the same molecular weight as the immunoprecipitate from watermelon cotyledons, indicating the lack of a cleavable extra sequence. The watermelon HPR translated in oocytes was imported into isolated glyoxysomes from castor bean (Ricinus communis L.) endosperm and remained resistant to proteolysis after the addition of proteinase K. The HPR did not change its apparent molecular weight during sequestration; however, it may have changed its conformation.
Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975, Pubmed
Blobel,
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
1975,
Pubmed
Feierabend,
Developmental studies on microbodies in wheat leaves : I. Conditions influencing enzyme development.
1972,
Pubmed
Fujiki,
Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes.
1984,
Pubmed
Gerdes,
Biosynthesis of a microbody matrix enzyme in greening cotyledons : Glycollate oxidase synthesized in vivo and in vitro.
1982,
Pubmed
Gietl,
Import of glyoxysomal malate dehydrogenase precursor into glyoxysomes: A heterologous in-vitro system.
1986,
Pubmed
Hock,
[Isoenzymes of malate dehydrogenase from watermelon seedlings: Disappearance of microheterogenity during germination].
1973,
Pubmed
Hock,
[Inhibition of isocitrate lyase in watermelon seedlings by white light].
1969,
Pubmed
Hondred,
Light-stimulated accumulation of the peroxisomal enzymes hydroxypyruvate reductase and serine:glyoxylate aminotransferase and their translatable mRNAs in cotyledons of cucumber seedlings.
1987,
Pubmed
Kagawa,
The development of microbodies (glyoxysomes and leaf peroxisomes) in cotyledons of germinating watermelon seedlings.
1975,
Pubmed
Kohn,
The kinetic properties of spinach leaf glyoxylic acid reductase.
1970,
Pubmed
Köster,
In vitro synthesis of peroxisomal membrane polypeptides.
1986,
Pubmed
Roberts,
Synthesis and posttranslational segregation of glyoxysomal isocitrate lyase from castor bean endosperm.
1981,
Pubmed
Rogers,
Lipid-protein interactions in the reconstitution of the microsomal reduced nicotinamide adenine dinucleotide-cytochrome b 5 reductase system.
1973,
Pubmed
Sautter,
Fluorescence immunohistochemical localization of malate dehydrogenase isoenzymes in watermelon cotyledons : a developmental study of glyoxysomes and mitochondria.
1982,
Pubmed
Sautter,
Microbody transition in greening watermelon cotyledons Double immunocytochemical labeling of isocitrate lyase and hydroxypyruvate reductase.
1986,
Pubmed
Sautter,
Glyoxysomal citrate synthase from watermelon cotyledons: immunocytochemical localization and heterologous translation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Schnarrenberger,
Development of Microbodies in Sunflower Cotyledons and Castor Bean Endosperm during Germination.
1971,
Pubmed
Titus,
Purification and characterization of hydroxypyruvate reductase from cucumber cotyledons.
1983,
Pubmed
Titus,
Investigation of the glyoxysome-peroxisome transition in germinating cucumber cotyledons using double-label immunoelectron microscopy.
1985,
Pubmed
Tokuyasu,
Improved procedures for immunoferritin labeling of ultrathin frozen sections.
1976,
Pubmed
Tolbert,
Localization and properties of hydroxypyruvate and glyoxylate reductases in spinach leaf particles.
1970,
Pubmed
Walk,
Cell-free synthesis of glyoxysomal malate dehydrogenase.
1978,
Pubmed
Warren,
Sulfhydryl studies of spinach leaf glyoxylic acid reductase.
1970,
Pubmed
Zimmermann,
Biogenesis of glyoxysomes. Synthesis and intracellular transfer of isocitrate lyase.
1980,
Pubmed
