Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Am J Physiol Regul Integr Comp Physiol March 1, 2014; 306 (5): R315-27.

Na+/H+ and Na+/NH+4 activities of zebrafish NHE3b expressed in Xenopus oocytes.

Ito Y , Kato A , Hirata T , Hirose S , Romero MF .

Zebrafish Na(+)/H(+) exchanger 3b (zNHE3b) is highly expressed in the apical membrane of ionocytes where Na(+) is absorbed from ion-poor fresh water against a concentration gradient. Much in vivo data indicated that zNHE3b is involved in Na(+) absorption but not leakage. However, zNHE3b-mediated Na(+) absorption has not been thermodynamically explained, and zNHE3b activity has not been measured. To address this issue, we overexpressed zNHE3b in Xenopus oocytes and characterized its activity by electrophysiology. Exposure of zNHE3b oocytes to Na(+)-free media resulted in significant decrease in intracellular pH (pH(i)) and intracellular Na(+) activity (aNa(i)). aNa(i) increased significantly when the cytoplasm was acidified by media containing CO₂-HCO₃(-) or butyrate. Activity of zNHE3b was inhibited by amiloride or 5-ethylisopropyl amiloride (EIPA). Although the activity was accompanied by a large hyperpolarization of ∼50 mV, voltage-clamp experiments showed that Na(+)/H(+) exchange activity of zNHE3b is electroneutral. Exposure of zNHE3b oocytes to medium containing NH₃/NH₄(+) resulted in significant decreases in pH(i) and aNa(i) and significant increase in intracellular NH₄(+) activity, indicating that zNHE3b mediates the Na(+)/NH₄(+) exchange. In low-Na(+) (0.5 mM) media, zNHE3b oocytes maintained aNa(i) of 1.3 mM, and Na(+)-influx was observed when pHi was decreased by media containing CO₂-HCO₃(-) or butyrate. These results provide thermodynamic evidence that zNHE3b mediates Na(+) absorption from ion-poor fresh water by its Na(+)/H(+) and Na(+)/NH₄(+) exchange activities.

PubMed ID: 24401990
PMC ID: PMC3949079
Article link: Am J Physiol Regul Integr Comp Physiol
Grant support: [+]
Genes referenced: btg3 slc9a3

References [+] :
Boisen, Sodium and chloride transport in soft water and hard water acclimated zebrafish (Danio rerio). 2004, Pubmed

Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.14.0
Major funding for Xenbase is provided by grant P41 HD064556