Srivastava S et al. (2013), Identification of compound heterozygous KCNJ1 m...

XB-ART-48338

Physiol Rep 2013 Nov 01;16:e00160. doi: 10.1002/phy2.160.

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Identification of compound heterozygous KCNJ1 mutations (encoding ROMK) in a kindred with Bartter's syndrome and a functional analysis of their pathogenicity.

Srivastava S , Li D , Edwards N , Hynes AM , Wood K , Al-Hamed M , Wroe AC , Reaich D , Moochhala SH , Welling PA , Sayer JA .

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A multiplex family was identified with biochemical and clinical features suggestive of Bartter's syndrome (BS). The eldest sibling presented with developmental delay and rickets at 4 years of age with evidence of hypercalciuria and hypokalemia. The second sibling presented at 1 year of age with urinary tract infections, polyuria, and polydipsia. The third child was born after a premature delivery with a history of polyhydramnios and neonatal hypocalcemia. Following corrective treatment she also developed hypercalciuria and a hypokalemic metabolic alkalosis. There was evidence of secondary hyperreninemia and hyperaldosteronism in all three siblings consistent with BS. Known BS genes were screened and functional assays of ROMK (alias KCNJ1, Kir1.1) were carried out in Xenopus oocytes. We detected compound heterozygous missense changes in KCNJ1, encoding the potassium channel ROMK. The S219R/L220F mutation was segregated from father and mother, respectively. In silico modeling of the missense mutations suggested deleterious changes. Studies in Xenopus oocytes revealed that both S219R and L220F had a deleterious effect on ROMK-mediated potassium currents. Coinjection to mimic the compound heterozygosity produced a synergistic decrease in channel function and revealed a loss of PKA-dependent stabilization of PIP2 binding. In conclusion, in a multiplex family with BS, we identified compound heterozygous mutations in KCNJ1. Functional studies of ROMK confirmed the pathogenicity of these mutations and defined the mechanism of channel dysfunction.

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Species referenced: Xenopus
Genes referenced: gnl3 kcnj1 kcnj12 she tbx2
GO keywords: potassium channel activity

???displayArticle.disOnts??? Bartter disease
???displayArticle.omims??? BARTTER SYNDROME, TYPE 2, ANTENATAL; BARTS2

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	Figure 1. Clinical and molecular genetic analysis of Bartter's syndrome patients. Hyperplasia of juxtaglomerular apparatus is demonstrated on renal histology in (A) sibling 1 and (B) sibling 2. (C) Renal USS demonstrates nephrocalcinosis in sibling 3. (D) KNCJ1 sequencing identifies compound heterozygous mutations in all three affected p.S219R/p.L220F segregating from each parent.
	Figure 2. Homology modeling of ROMK. (A) Crystal structure of chicken Kcnj12/Kir2.2 (Hansen et al. 2011). One of the four Kcnj12 monomers forming the potassium channel is highlighted in orange. (B) Putative structure of the ROMK monomer (green) compared with chicken Kcnj12 (orange). The black box denotes the position of S219 and L220 in ROMK. (C) Partial amino acid sequence alignment of the human KCNJ family. (D) Putative position of S219 and L220 in ROMK and the homologous residues in chicken Kcnj12 (S221 and H222, respectively).
	Figure 3. Comparison of the properties of wild-type (WT) and mutant ROMK channels in Xenopus oocytes. Shown are families of whole-cell currents in oocytes injected with ROMK cRNA-encoding WT, mutant cRNA (S219R and L220F), or combinations of both to mimic heterozygosity and compound heterozygosity. Oocytes were held at −60 mV and clamped in 20 mV increments from −120 mV to +60 mV in 20 mV steps (5 mmol/L potassium).
	Figure 4. Effect of S219R and L220F mutations on ROMK channel activity. Relative outward potassium currents recorded by two-electrode voltage -clamp in Xenopus oocytes expressing ROMK. Oocytes were injected with 250 pg cRNA-encoding wild type (WT), S219R, or L220F mutant channel subunits. To mimic the compound heterozygous state of the patients, oocytes were coinjected with mixtures (given in parentheses) of the different cRNAs (250 pg in total). Data are mean ± SEM (n = 18 oocytes/group, from three frogs). *P < 0.001; P < 0.01; ns, P > 0.05 versus WT potassium current. #P < 0.001 versus WT+S219R current.
	Figure 5. L220F affects phosphatidylinositol 4,5-bisphosphate (PIP2)-dependent gating of ROMK. Whole-cell potassium currents recorded in Xenopus oocytes coexpressing the M1 receptor with ROMK channels comprising wild type (WT) and/or L220F mutant subunits. Relative doses are given in parentheses. Data are expressed as the percent inhibition of the potassium current induced by exposure (10 min) to carbachol (25 μmol/L) and are mean ± SEM (n = 12–15 oocytes/group, from three frogs). ***P < 0.001; ns, P > 0.05 versus inhibition of WT potassium current.

References [+] :

Adzhubei, A method and server for predicting damaging missense mutations. 2010, Pubmed