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XB-ART-4834
Dev Biol August 15, 2003; 260 (2): 449-64.

Integrin alpha5beta1 supports the migration of Xenopus cranial neural crest on fibronectin.

Alfandari D , Cousin H , Gaultier A , Hoffstrom BG , DeSimone DW .


Abstract
During early embryonic development, cranial neural crest cells emerge from the developing mid- and hindbrain. While numerous studies have focused on integrin involvement in trunk neural crest cell migration, comparatively little is known about mechanisms of cranial neural crest cell migration. We show that fibronectin, but not laminin, vitronectin, or type I collagen can support cranial neural crest cell migration and segmentation in vitro. These behaviors require both the RGD and "synergy" sites located within the central cell-binding domain of fibronectin. While these two sites are sufficient for cranial neural crest cell migration, we find that the second Heparin-binding domain of fibronectin can provide additional support for cranial neural crest cell migration in vitro. Finally, using a function blocking monoclonal antibody, we show that cranial neural crest cell migration on fibronectin requires the integrin alpha5beta1.

PubMed ID: 12921745
Article link: Dev Biol
Grant support: [+]
Genes referenced: fn1
Antibodies: Itgb1 Ab3



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