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XB-ART-48374
PLoS One January 1, 2013; 8 (12): e84717.

Protein tyrosine phosphatase 4A3 (PTP4A3) is required for Xenopus laevis cranial neural crest migration in vivo.

Maacha S , Planque N , Laurent C , Pegoraro C , Anezo O , Maczkowiak F , Monsoro-Burq AH , Saule S .


Abstract
Uveal melanoma is the most common intraocular malignancy in adults, representing between about 4% and 5% of all melanomas. High expression levels of Protein Tyrosine Phosphatase 4A3, a dual phosphatase, is highly predictive of metastasis development and PTP4A3 overexpression in uveal melanoma cells increases their in vitro migration and in vivo invasiveness. Melanocytes, including uveal melanocytes, are derived from the neural crest during embryonic development. We therefore suggested that PTP4A3 function in uveal melanoma metastasis may be related to an embryonic role during neural crest cell migration. We show that PTP4A3 plays a role in cephalic neural crest development in Xenopus laevis. PTP4A3 loss of function resulted in a reduction of neural crest territory, whilst gain of function experiments increased neural crest territory. Isochronic graft experiments demonstrated that PTP4A3-depleted neural crest explants are unable to migrate in host embryos. Pharmacological inhibition of PTP4A3 on dissected neural crest cells significantly reduced their migration velocity in vitro. Our results demonstrate that PTP4A3 is required for cephalic neural crest migration in vivo during embryonic development.

PubMed ID: 24376839
PMC ID: PMC3871671
Article link: PLoS One

Genes referenced: casp3.2 h2bc21 ocm1 prl.2 ptp4a3 slc12a3 twist1
Antibodies: Casp3 Ab1 H3f3a Ab9
Morpholinos: ptp4a3 MO1


Article Images: [+] show captions
References [+] :
Aceto, Tyrosine phosphatase SHP2 promotes breast cancer progression and maintains tumor-initiating cells via activation of key transcription factors and a positive feedback signaling loop. 2012, Pubmed


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