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XB-ART-48484
PLoS One January 1, 2014; 9 (1): e86784.

Plakophilin-3 catenin associates with the ETV1/ER81 transcription factor to positively modulate gene activity.

Munoz WA , Lee M , Miller RK , Ahmed Z , Ji H , Link TM , Lee GR , Kloc M , Ladbury JE , McCrea PD .


Abstract
Members of the plakophilin-catenin sub-family (Pkp-1, -2, and -3) facilitate the linkage of desmosome junctional components to each other (e.g. desmosomal cadherins to desmoplakin) and the intermediate-filament cytoskeleton. Pkps also contribute to desmosomal stabilization and the trafficking of its components. The functions of Pkps outside of the desmosome are less well studied, despite evidence suggesting their roles in mRNA regulation, small-GTPase modulation (e.g. mid-body scission) during cell division, and cell survival following DNA damage. Pkp-catenins are further believed to have roles in the nucleus given their nuclear localization in some contexts and the known nuclear roles of structurally related catenins, such as beta-catenin and p120-catenin. Further, Pkp-catenin activities in the nuclear compartment have become of increased interest with the identification of interactions between Pkp2-catenin and RNA Pol III and Pkp1 with single-stranded DNA. Consistent with earlier reports suggesting possible nuclear roles in development, we previously demonstrated prominent nuclear localization of Pkp3 in Xenopus naïve ectoderm ("animal cap") cells and recently resolved a similar localization in mouse embryonic stem cells. Here, we report the association and positive functional interaction of Pkp3 with a transcription factor, Ets variant gene 1 (ETV1), which has critical roles in neural development and prominent roles in human genetic disease. Our results are the first to report the interaction of a sequence-specific transcription factor with any Pkp. Using Xenopus laevis embryos and mammalian cells, we provide evidence for the Pkp3:ETV1 complex on both biochemical and functional levels.

PubMed ID: 24475179
PMC ID: PMC3903613
Article link: PLoS One
Grant support: [+]
Genes referenced: arvcf ctnnd1 ddc etv1 fes foxd3 mmp1 myc pigy pkp1 pkp2 pkp3 twist1
Antibodies: Etv1 Ab1 HA Ab6 Myc Ab3 Myc Ab6 Pkp3 Ab1 Pkp3 Ab2
Morpholinos: pkp3 MO1


Article Images: [+] show captions
References:
Abe, 2011, Pubmed [+]


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