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Nat Commun July 8, 2014; 5 3318.

Phosphorylation of ARPP19 by protein kinase A prevents meiosis resumption in Xenopus oocytes.

Dupré A , Daldello EM , Nairn AC , Jessus C , Haccard O .

During oogenesis, oocytes are arrested in prophase and resume meiosis by activating the kinase Cdk1 upon hormonal stimulation. In all vertebrates, release from prophase arrest relies on protein kinase A (PKA) downregulation and on the dephosphorylation of a long sought but still unidentified substrate. Here we show that ARPP19 is the PKA substrate whose phosphorylation at serine 109 is necessary and sufficient for maintaining Xenopus oocytes arrested in prophase. By downregulating PKA, progesterone, the meiotic inducer in Xenopus, promotes partial dephosphorylation of ARPP19 that is required for the formation of a threshold level of active Cdk1. Active Cdk1 then initiates the MPF autoamplification loop that occurs independently of both PKA and ARPP19 phosphorylation at serine 109 but requires the Greatwall (Gwl)-dependent phosphorylation of ARPP19 at serine 67. Therefore, ARPP19 stands at a crossroads in the meiotic M-phase control network by integrating differential effects of PKA and Gwl, two kinases essential for meiosis resumption.

PubMed ID: 24525567
PMC ID: PMC4014304
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: ankrd2 arpp19 ccnb1.2 ccnb2 cdc27 cdk1 lyz mapk1 mastl mos pkmyt1 plk1 ppp2r2d
Antibodies: Mos Ab2

Article Images: [+] show captions
References [+] :
Abrieu, The Polo-like kinase Plx1 is a component of the MPF amplification loop at the G2/M-phase transition of the cell cycle in Xenopus eggs. 1999, Pubmed, Xenbase