Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-48560
Nucleic Acids Res April 1, 2014; 42 (8): 5059-71.

Selective nuclear export of specific classes of mRNA from mammalian nuclei is promoted by GANP.

Wickramasinghe VO , Andrews R , Ellis P , Langford C , Gurdon JB , Stewart M , Venkitaraman AR , Laskey RA .


Abstract
The nuclear phase of the gene expression pathway culminates in the export of mature messenger RNAs (mRNAs) to the cytoplasm through nuclear pore complexes. GANP (germinal- centre associated nuclear protein) promotes the transfer of mRNAs bound to the transport factor NXF1 to nuclear pore complexes. Here, we demonstrate that GANP, subunit of the TRanscription-EXport-2 (TREX-2) mRNA export complex, promotes selective nuclear export of a specific subset of mRNAs whose transport depends on NXF1. Genome-wide gene expression profiling showed that half of the transcripts whose nuclear export was impaired following NXF1 depletion also showed reduced export when GANP was depleted. GANP-dependent transcripts were highly expressed, yet short-lived, and were highly enriched in those encoding central components of the gene expression machinery such as RNA synthesis and processing factors. After injection into Xenopus oocyte nuclei, representative GANP-dependent transcripts showed faster nuclear export kinetics than representative transcripts that were not influenced by GANP depletion. We propose that GANP promotes the nuclear export of specific classes of mRNAs that may facilitate rapid changes in gene expression.

PubMed ID: 24510098
PMC ID: PMC4005691
Article link: Nucleic Acids Res
Grant support: [+]
Genes referenced: actl6a ankrd2 arpp19 axin2 eny2 exosc6 galk1 idh2 nxf1 pcid2 rps23 sp1 tpr tst


Article Images: [+] show captions
References [+] :
Audibert, In vivo kinetics of mRNA splicing and transport in mammalian cells. 2002, Pubmed


Xenbase: The Xenopus Model Organism Knowledgebase.
Version: 4.16.0
Major funding for Xenbase is provided by grant P41 HD064556