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Biophys J
2014 Feb 04;1063:735-40. doi: 10.1016/j.bpj.2013.12.033.
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Micromechanics of the vertebrate meiotic spindle examined by stretching along the pole-to-pole axis.
Takagi J
,
Itabashi T
,
Suzuki K
,
Shimamoto Y
,
Kapoor TM
,
Ishiwata S
.
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The meiotic spindle is a bipolar molecular machine that is designed to segregate duplicated chromosomes toward the opposite poles of the cell. The size and shape of the spindle are considered to be maintained by a balance of forces produced by molecular motors and microtubule assembly dynamics. Several studies have probed how mechanical perturbations of the force balance affect the spindle structure. However, the spindle's response to a stretching force acting at the spindle pole and along its long axis, i.e., the direction in which chromosomes are segregated, has not been examined. Here, we describe a method to apply a stretching force to the metaphase spindle assembled in Xenopus egg extracts and measure the relationship between the force and the three-dimensional deformation of the spindle. We found that the spindle behaves as a Zener-type viscoelastic body when forces are applied at the spindle pole, generating a restoring force for several minutes. In addition, both the volume of the spindle and the tubulin density are conserved under the stretching force. These results provide insight into how the spindle size is maintained at metaphase.
Brust-Mascher,
Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.
2009, Pubmed
Brust-Mascher,
Kinesin-5-dependent poleward flux and spindle length control in Drosophila embryo mitosis.
2009,
Pubmed
Burbank,
Slide-and-cluster models for spindle assembly.
2007,
Pubmed
,
Xenbase
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Dumont,
Compression regulates mitotic spindle length by a mechanochemical switch at the poles.
2009,
Pubmed
Gatlin,
Directly probing the mechanical properties of the spindle and its matrix.
2010,
Pubmed
,
Xenbase
Goshima,
Length control of the metaphase spindle.
2005,
Pubmed
Grill,
The distribution of active force generators controls mitotic spindle position.
2003,
Pubmed
Grill,
Spindle positioning by cortical pulling forces.
2005,
Pubmed
Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Hyman,
Preparation of modified tubulins.
1991,
Pubmed
Itabashi,
Probing the mechanical architecture of the vertebrate meiotic spindle.
2009,
Pubmed
,
Xenbase
Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Lynch,
Effect of fiber orientation and strain rate on the nonlinear uniaxial tensile material properties of tendon.
2003,
Pubmed
Millman,
The filament lattice of striated muscle.
1998,
Pubmed
Minc,
Influence of cell geometry on division-plane positioning.
2011,
Pubmed
Ofek,
Contribution of the cytoskeleton to the compressive properties and recovery behavior of single cells.
2009,
Pubmed
Oguchi,
The bidirectional depolymerizer MCAK generates force by disassembling both microtubule ends.
2011,
Pubmed
Petry,
Branching microtubule nucleation in Xenopus egg extracts mediated by augmin and TPX2.
2013,
Pubmed
,
Xenbase
Salmon,
Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching.
1984,
Pubmed
Shimamoto,
Insights into the micromechanical properties of the metaphase spindle.
2011,
Pubmed
,
Xenbase
Shimamoto,
Microneedle-based analysis of the micromechanics of the metaphase spindle assembled in Xenopus laevis egg extracts.
2012,
Pubmed
,
Xenbase
Takagi,
Using micromanipulation to analyze control of vertebrate meiotic spindle size.
2013,
Pubmed
,
Xenbase
Théry,
Experimental and theoretical study of mitotic spindle orientation.
2007,
Pubmed
Tirnauer,
Microtubule plus-end dynamics in Xenopus egg extract spindles.
2004,
Pubmed
,
Xenbase
Wollman,
Reverse engineering of force integration during mitosis in the Drosophila embryo.
2008,
Pubmed
