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XB-ART-48580
Mech Dev May 1, 2014; 132 28-37.

RFX7 is required for the formation of cilia in the neural tube.

Manojlovic Z , Earwood R , Kato A , Stefanovic B , Kato Y .


Abstract
Regulatory Factor X (RFX) transcription factors are important for development and are likely involved in the pathogenesis of serious human diseases including ciliopathies. While seven RFX genes have been identified in vertebrates and several RFX transcription factors have been reported to be regulators of ciliogenesis, the role of RFX7 in development including ciliogenesis is not known. Here we show that RFX7 in Xenopus laevis is expressed in the neural tube, eye, otic vesicles, and somites. Knockdown of RFX7 in Xenopus embryos resulted in a defect of ciliogenesis in the neural tube and failure of neural tube closure. RFX7 controlled the formation of cilia by regulating the expression of RFX4 gene, which has been reported to be required for ciliogenesis in the neural tube. Moreover, ectopic expression of Foxj1, which is a master regulator of motile cilia formation, suppressed the expression of RFX4 but not RFX7. Taken together, RFX7 plays an important role in the process of neural tube closure at the top of the molecular cascade which controls ciliogenesis in the neural tube.

PubMed ID: 24530844
PMC ID: PMC3976564
Article link: Mech Dev
Grant support: [+]
Genes referenced: arl13b foxj1 foxj1.2 gal.2 mcidas rfx4 rfx7 tuba4b tubal3.1
GO keywords: neural tube closure [+]
Antibodies: Arl13b Ab2 FLAG Ab1 HA Ab2 Tuba4b Ab4
Morpholinos: rfx7 MO1 rfx7 MO2

Disease Ontology terms: ciliopathy
OMIMs: NEURAL TUBE DEFECTS, SUSCEPTIBILITY TO; NTD

Article Images: [+] show captions
References:
Aftab, 2008, Pubmed [+]


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