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XB-ART-4863
Biochem Biophys Res Commun. August 29, 2003; 308 (3): The isolation and characterization of XC3H-3b: a CCCH zinc-finger protein required for pronephros development.
Kaneko T
,
Chan T
,
Satow R
,
Fujita T
,
Asashima M
.
Abstract We describe the isolation and characterization of the RNA-binding protein XC3H-3b that is expressed during pronephros development. XC3H-3b is a member of the TTP/TIS family of CCCH tandem zinc-finger proteins, which are physiological stimulators of instability for the mRNA encoding tumor necrosis factor-alpha in certain cell types. XC3H-3b is localized primarily to the mesodermal tissues around the pronephros. Overexpression of XC3H-3b markedly and specifically inhibits kidney development. Morpholino-mediated knockdown of XC3H-3b also results in defects in nephrogenesis. In both cases, the expression of numerous pronephric marker genes, such as Xlim-1, Xpax-2, Xpax-8, Xwnt-4, and XWT1, is decreased and morphological development of the pronephric tubules is abrogated. We conclude that XC3H-3b plays an important role in the regulation of pronephros differentiation. This is the first report of a gene localized around the pronephros that regulates pronephros development.Pubmed Id: 12914788 Article link: Biochem Biophys Res Commun.Genes referenced: lhx1 pax2 pax8 sh3bp4 wnt4 wt1 zfp36 zfp36l2 Antibodies referenced: |
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