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XB-ART-48686
Sci Rep February 26, 2014; 4 4365.

Quantitative proteomics of Xenopus laevis embryos: expression kinetics of nearly 4000 proteins during early development.

Sun L , Bertke MM , Champion MM , Zhu G , Huber PW , Dovichi NJ .


Abstract
While there is a rich literature on transcription dynamics during the development of many organisms, protein data is limited. We used iTRAQ isotopic labeling and mass spectrometry to generate the largest developmental proteomic dataset for any animal. Expression dynamics of nearly 4,000 proteins of Xenopus laevis was generated from fertilized egg to neurula embryo. Expression clusters into groups. The cluster profiles accurately reflect the major events that mark changes in gene expression patterns during early Xenopus development. We observed decline in the expression of ten DNA replication factors after the midblastula transition (MBT), including a marked decline of the licensing factor XCdc6. Ectopic expression of XCdc6 leads to apoptosis; temporal changes in this protein are critical for proper development. Measurement of expression in single embryos provided no evidence for significant protein heterogeneity between embryos at the same stage of development.

PubMed ID: 24626130
PMC ID: PMC3953746
Article link: Sci Rep
Grant support: [+]
Genes referenced: ccdc6 cdc6 diaph1 h4c4 khsrp mcm3 mcm5 mcm6 mcm7 myo10 orc1 orc5 prss1 psmd6 recql4 spock2 ticrr


Article Images: [+] show captions
References [+] :
Abiko, Identification of proteins enriched in rice egg or sperm cells by single-cell proteomics. 2013, Pubmed


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