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XB-ART-48717
Nat Commun July 8, 2014; 5 3516.

EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1.

Ji YJ , Hwang YS , Mood K , Cho HJ , Lee HS , Winterbottom E , Cousin H , Daar IO .


Abstract
The Eph/ephrin signalling pathways have a critical function in cell adhesion and repulsion, and thus play key roles in various morphogenetic events during development. Here we show that a decrease in ephrinB2 protein causes neural tube closure defects during Xenopus laevis embryogenesis. Such a decrease in ephrinB2 protein levels is observed on the loss of flotillin-1 scaffold protein, a newly identified ephrinB2-binding partner. This dramatic decline in ephrinB2 protein levels on the absence of flotillin-1 expression is specific, and is partly the result of an increased susceptibility to cleavage by the metalloprotease ADAM10. These findings indicate that flotillin-1 regulates ephrinB2 protein levels through ADAM10, and is required for appropriate neural tube morphogenesis in the Xenopus embryo.

PubMed ID: 24662724
PMC ID: PMC4120273
Article link: Nat Commun
Grant support: [+]
Genes referenced: actl6a adam10 adam17 efnb1 efnb2 efnb3 flot1 flot2 igf2bp3 isyna1 mapk1
Morpholinos: adam10 MO1 adam17 MO1 adam33 MO4 efnb2 MO2 flot1 MO1 flot1 MO2


Article Images: [+] show captions
References:
Adams, 1999, Pubmed [+]


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