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Figure 5. Time-lapse imaging of hindlimb development. A–C: Growth of a stage 53–54 lefthindlimb at 24 hr intervals over 48 hr. During this time melanophores can be seen migrating and expanding on the ventral edge of the left hind limb (arrowheads indicate the expanding range of these cells), in addition to vascularization and digit development in the digital plate. The shape of the marginal vein can be seen changing as digit 4 elongates (asterisk indicates the location of digit 4). A dashed line has been included as a reference mark for this elongation. This plate corresponds to Supp. Movie S1. Relative time is denoted in hours:minutes. Scale bar = 250 μm in A.Download figure to PowerPoint
Figure 6. Micrographs of cell division in Xenopus corneaepithelium. A: The mG-HGEM transgenic construct carrying a heat-shock inducible histone H2B-mCherry protein. It also carries a membrane GFP protein separated by a 2A peptide sequence (GFP expression was not imaged in these experiments). As a screening tool the construct uses the lens-specific gamma-crystallin promoter to drive GFP expression. B–G: Imaging H2B-mCherry expression in a cornea epithelial cell going through mitosis (arrowheads). (B) Premitotic cell. (C) Prophase. (D) Metaphase. (E) Anaphase. (F) Telophase. (G) The resulting daughter cells. This particular division occurred over an unusually long distance. A movie of this division can be seen in Supp. Movie S2. Relative time is denoted in hours:minutes. Scale bar = 20 μm in G.
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