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XB-ART-48872
Development. May 1, 2014; 141 (9): 1927-39.

High-resolution analysis of gene activity during the Xenopus mid-blastula transition.

Collart C , Owens ND , Bhaw-Rosun L , Cooper B , De Domenico E , Patrushev I , Sesay AK , Smith JN , Smith JC , Gilchrist MJ .


Abstract
The Xenopus mid-blastula transition (MBT) marks the onset of large-scale zygotic transcription, as well as an increase in cell cycle length and a loss of synchronous cell divisions. Little is known about what triggers the activation of transcription or how newly expressed genes interact with each other. Here, we use high-resolution expression profiling to identify three waves of gene activity: a post-fertilisation wave involving polyadenylation of maternal transcripts; a broad wave of zygotic transcription detectable as early as the seventh cleavage and extending beyond the MBT at the twelfth cleavage; and a shorter post-MBT wave of transcription that becomes apparent as development proceeds. Our studies have also allowed us to define a set of maternal mRNAs that are deadenylated shortly after fertilisation, and are likely to be degraded thereafter. Experimental analysis indicates that the polyadenylation of maternal transcripts is necessary for the establishment of proper levels of zygotic transcription at the MBT, and that genes activated in the second wave of expression, including Brachyury and Mixer, contribute to the regulation of genes expressed in the third. Together, our high-resolution time series and experimental studies have yielded a deeper understanding of the temporal organisation of gene regulatory networks in the early Xenopus embryo.

PubMed ID: 24757007
PMC ID: PMC3994770
Article link: Development.
Grant support: A252-5RG50 Medical Research Council , A252-5RG70 Medical Research Council , MC_U117597140 Medical Research Council , MC_U117597137 Medical Research Council , MC_U117597137 Medical Research Council , MC_U117597140 Medical Research Council , A252-5RG50 Medical Research Council , A252-5RG70 Medical Research Council , MRC_MC_U117597137 Medical Research Council , MRC_MC_U117597140 Medical Research Council , A252-5RG50 Medical Research Council , A252-5RG70 Medical Research Council , MC_U117597137 Medical Research Council , MC_U117597140 Medical Research Council , A252-5RG50 Medical Research Council , A252-5RG70 Medical Research Council

Genes referenced: adcy3 aplnr bix1.1 bmp4 ccnb3 cer1 cog8 dpp8 eef1a1 ehmt1 eno1 eomes fam46c flrt2 fmn2 foxi4.2 gata5 gdf3 gtf2ird1 hmg20b hs3st1 hspa8 ints12 kif9 kifap3 klf17 lekr1 lhx5 lrr1 mapk4 mixer mixl1 mn1 msgn1 mybl2 myf5 ninl nodal3.1 nodal6 nol8 not nup155 odc1 pak4 plod2 rasl11b ric3 sox17a ssh3 t tbx2 tbxa2r tfap2a ube2s vegt ventx1.2 znf574

Morpholinos referenced: eomes MO1 eomes MO2 mixer MO4 t MO2 t MO3 t2 MO2 t2 MO3 vegt MO3

References:
Aanes, 2011, Pubmed[+]


Article Images: [+] show captions

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