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Nat Struct Mol Biol
2014 Mar 01;213:244-52. doi: 10.1038/nsmb.2768.
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Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Li Q
,
Wanderling S
,
Paduch M
,
Medovoy D
,
Singharoy A
,
McGreevy R
,
Villalba-Galea CA
,
Hulse RE
,
Roux B
,
Schulten K
,
Kossiakoff A
,
Perozo E
.
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The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing domains (VSDs) carry out these functions through reorientations of positive charges in the S4 helix. Here, we determined crystal structures of the Ciona intestinalis VSD (Ci-VSD) in putatively active and resting conformations. S4 undergoes an ~5-Å displacement along its main axis, accompanied by an ~60° rotation. This movement is stabilized by an exchange in countercharge partners in helices S1 and S3 that generates an estimated net charge transfer of ~1 eo. Gating charges move relative to a ''hydrophobic gasket' that electrically divides intra- and extracellular compartments. EPR spectroscopy confirms the limited nature of S4 movement in a membrane environment. These results provide an explicit mechanism for voltage sensing and set the basis for electromechanical coupling in voltage-dependent enzymes and ion channels.
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010, Pubmed
Adams,
PHENIX: a comprehensive Python-based system for macromolecular structure solution.
2010,
Pubmed
Ahern,
Focused electric field across the voltage sensor of potassium channels.
2005,
Pubmed
,
Xenbase
Armstrong,
Currents related to movement of the gating particles of the sodium channels.
1973,
Pubmed
Arrigoni,
The voltage-sensing domain of a phosphatase gates the pore of a potassium channel.
2013,
Pubmed
,
Xenbase
Bezanilla,
How membrane proteins sense voltage.
2008,
Pubmed
Bezanilla,
Gating currents.
1998,
Pubmed
Campos,
Two atomic constraints unambiguously position the S4 segment relative to S1 and S2 segments in the closed state of Shaker K channel.
2007,
Pubmed
,
Xenbase
Catterall,
Molecular properties of voltage-sensitive sodium channels.
1986,
Pubmed
Cha,
Characterizing voltage-dependent conformational changes in the Shaker K+ channel with fluorescence.
1997,
Pubmed
,
Xenbase
Cha,
Atomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy.
1999,
Pubmed
Chen,
MolProbity: all-atom structure validation for macromolecular crystallography.
2010,
Pubmed
Emsley,
Coot: model-building tools for molecular graphics.
2004,
Pubmed
Fellouse,
High-throughput generation of synthetic antibodies from highly functional minimalist phage-displayed libraries.
2007,
Pubmed
Glauner,
Spectroscopic mapping of voltage sensor movement in the Shaker potassium channel.
1999,
Pubmed
,
Xenbase
Goldstein,
A structural vignette common to voltage sensors and conduction pores: canaliculi.
1996,
Pubmed
Guy,
Molecular model of the action potential sodium channel.
1986,
Pubmed
HODGKIN,
A quantitative description of membrane current and its application to conduction and excitation in nerve.
1952,
Pubmed
Henrion,
Tracking a complete voltage-sensor cycle with metal-ion bridges.
2012,
Pubmed
,
Xenbase
Hobiger,
Coupling of Ci-VSP modules requires a combination of structure and electrostatics within the linker.
2012,
Pubmed
,
Xenbase
Jensen,
Mechanism of voltage gating in potassium channels.
2012,
Pubmed
Jiang,
The principle of gating charge movement in a voltage-dependent K+ channel.
2003,
Pubmed
Jiang,
X-ray structure of a voltage-dependent K+ channel.
2003,
Pubmed
Koag,
Voltage-dependent conformational changes of KVAP S4 segment in bacterial membrane environment.
2009,
Pubmed
Kohout,
Electrochemical coupling in the voltage-dependent phosphatase Ci-VSP.
2010,
Pubmed
Kohout,
Subunit organization and functional transitions in Ci-VSP.
2008,
Pubmed
Li,
Expression, purification, and reconstitution of the voltage-sensing domain from Ci-VSP.
2012,
Pubmed
Lin,
Transfer of ion binding site from ether-a-go-go to Shaker: Mg2+ binds to resting state to modulate channel opening.
2010,
Pubmed
,
Xenbase
Long,
Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment.
2007,
Pubmed
Long,
Crystal structure of a mammalian voltage-dependent Shaker family K+ channel.
2005,
Pubmed
Mannuzzu,
Direct physical measure of conformational rearrangement underlying potassium channel gating.
1996,
Pubmed
,
Xenbase
Murata,
Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor.
2005,
Pubmed
,
Xenbase
Murshudov,
Refinement of macromolecular structures by the maximum-likelihood method.
1997,
Pubmed
Payandeh,
The crystal structure of a voltage-gated sodium channel.
2011,
Pubmed
Posson,
Extent of voltage sensor movement during gating of shaker K+ channels.
2008,
Pubmed
,
Xenbase
Ramsey,
A voltage-gated proton-selective channel lacking the pore domain.
2006,
Pubmed
Ramu,
Enzymatic activation of voltage-gated potassium channels.
2006,
Pubmed
Rizk,
Allosteric control of ligand-binding affinity using engineered conformation-specific effector proteins.
2011,
Pubmed
Roux,
The membrane potential and its representation by a constant electric field in computer simulations.
2008,
Pubmed
Ruta,
Calibrated measurement of gating-charge arginine displacement in the KvAP voltage-dependent K+ channel.
2005,
Pubmed
Sasaki,
A voltage sensor-domain protein is a voltage-gated proton channel.
2006,
Pubmed
Schmidt,
Phospholipids and the origin of cationic gating charges in voltage sensors.
2006,
Pubmed
Tao,
A gating charge transfer center in voltage sensors.
2010,
Pubmed
,
Xenbase
Tombola,
How does voltage open an ion channel?
2006,
Pubmed
Trabuco,
Flexible fitting of atomic structures into electron microscopy maps using molecular dynamics.
2008,
Pubmed
Trabuco,
Molecular dynamics flexible fitting: a practical guide to combine cryo-electron microscopy and X-ray crystallography.
2009,
Pubmed
Vargas,
In search of a consensus model of the resting state of a voltage-sensing domain.
2011,
Pubmed
Villalba-Galea,
S4-based voltage sensors have three major conformations.
2008,
Pubmed
Villalba-Galea,
Sensing charges of the Ciona intestinalis voltage-sensing phosphatase.
2013,
Pubmed
Yang,
Molecular basis of charge movement in voltage-gated sodium channels.
1996,
Pubmed
Zhang,
Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.
2012,
Pubmed
Zheng,
Lipid-dependent gating of a voltage-gated potassium channel.
2011,
Pubmed
