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Dev Dyn October 1, 2014; 243 (10): 1352-61.

Characterization of the Rx1-dependent transcriptome during early retinal development.

Giudetti G , Giannaccini M , Biasci D , Mariotti S , Degl'innocenti A , Perrotta M , Barsacchi G , Andreazzoli M .

BACKGROUND: The transcription factor Rx1, also known as Rax, controls key properties of retinal precursors including migration behavior, proliferation, and maintenance of multipotency. However, Rx1 effector genes are largely unknown. RESULTS: To identify genes controlled by Rx1 in early retinal precursors, we compared the transcriptome of Xenopus embryos overexpressing Rx1 to that of embryos in which Rx1 was knocked-down. In particular, we selected 52 genes coherently regulated, i.e., actived in Rx1 gain of function and repressed in Rx1 loss of function experiments, or vice versa. RT-qPCR and in situ hybridization confirmed the trend of regulation predicted by microarray data for the selected genes. Most of the genes upregulated by Rx1 are coexpressed with this transcription factor, while downregulated genes are either not expressed or expressed at very low levels in the early developing retina. Putative direct Rx1 target genes, activated by GR-Rx1 in the absence of protein synthesis, include Ephrin B1 and Sh2d3c, an interactor of ephrinB1 receptor, which represent candidate novel effectors for the migration promoting activity of Rx1. CONCLUSIONS: This study identifies previously undescribed Rx1 regulated genes mainly involved in transcription regulation, cell migration/adhesion, and cell proliferation that contribute to delineate the molecular mechanisms underlying Rx1 activities.

PubMed ID: 24801179
Article link: Dev Dyn

Species referenced: Xenopus
Genes referenced: atrx bahcc1 bzw2 ccdc12 chpf crx ddr1 dgcr2 efnb1 eif4g1 enox1 fat1 fli1 gpatch8 gtpbp1 hey1 kdm7a kif26a med12 mef2d mrps5 myod1 myos ncoa6 nek2 notch1 p4ha1 p4ha2 pfdn6 polr2a ppfia1 psme1 rab11a rab11fip4 rab6a rax rbfox2 rbm24 rnf19a rpl9 scaf4 sh2d3c slc25a17 strn3 tcf3 tcf7l1 tcp1 thg1l tle2 tob2 trip12
Morpholinos: rax MO2

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