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Dev Cell April 28, 2014; 29 (2): 250-7.

Polarized Wnt signaling regulates ectodermal cell fate in Xenopus.

Huang YL , Niehrs C .

How cells convert polarity cues into cell fate specification is incompletely understood. Here, we show that Wnt/β-catenin and Wnt/PCP signaling cooperate in this process in early Xenopus embryos. We find that the Wnt coreceptor Lrp6 is asymmetrically localized to the basolateral membrane in ectodermal blastomeres. Lrp6 asymmetry is controlled by Wnt/PCP signaling, indicating that this pathway regulates not only planar- but also apicobasal cell polarity. Following asymmetric cell division, Lrp6 preferentially sorts to the deep ectodermal cell layer and becomes depleted in the epithelial cell layer. This is accompanied by elevated Wnt/β-catenin signaling in deep cells, which in turn promotes their differentiation into ciliated cells. We conclude that coordinated Wnt/PCP and Wnt/β-catenin signaling convert apicobasal polarity information to specify ectodermal cell fate.

PubMed ID: 24780739
Article link: Dev Cell

Genes referenced: axin2 ctnnb1 dvl2 fzd7 hes5.10 inka1 krt12.4 krt70 lrp6 pcdh8 pcdh8.2 sia1 tuba4b
Morpholinos: ctnnb1 MO1 dvl2 MO1 fzd7 MO1 lrp6 MO1 wnt5a MO1

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