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XB-ART-49287
J Cell Biol August 4, 2014; 206 (3): 367-76.

Radial intercalation is regulated by the Par complex and the microtubule-stabilizing protein CLAMP/Spef1.

Werner ME , Mitchell JW , Putzbach W , Bacon E , Kim SK , Mitchell BJ .


Abstract
The directed movement of cells is critical for numerous developmental and disease processes. A developmentally reiterated form of migration is radial intercalation; the process by which cells move in a direction orthogonal to the plane of the tissue from an inner layer to an outer layer. We use the radial intercalation of cells into the skin of Xenopus laevis embryos as a model to study directed cell migration within an epithelial tissue. We identify a novel function for both the microtubule-binding protein CLAMP and members of the microtubule-regulating Par complex during intercalation. Specifically, we show that Par3 and aPKC promote the apical positioning of centrioles, whereas CLAMP stabilizes microtubules along the axis of migration. We propose a model in which the Par complex defines the orientation of apical migration during intercalation and in which subcellular localization of CLAMP promotes the establishment of an axis of microtubule stability required for the active migration of cells into the outer epithelium.

PubMed ID: 25070955
PMC ID: PMC4121976
Article link: J Cell Biol
Grant support: [+]
Genes referenced: dnai1 mcc nhs pard3 prkci rpe slc4a1 spef1 tub
Antibodies: Spef1 Ab1
Morpholinos: pard3 MO2 spef1 MO1


Article Images: [+] show captions
References [+] :
Breuzard, Molecular mechanisms of Tau binding to microtubules and its role in microtubule dynamics in live cells. 2013, Pubmed


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