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XB-ART-49376
J Cell Biol August 18, 2014; 206 (4): 473-83.

cPKC regulates interphase nuclear size during Xenopus development.

Edens LJ , Levy DL .


Abstract
Dramatic changes in cell and nuclear size occur during development and differentiation, and aberrant nuclear size is associated with many disease states. However, the mechanisms that regulate nuclear size are largely unknown. A robust system for investigating nuclear size is early Xenopus laevis development, during which reductions in nuclear size occur without changes in DNA content. To identify cellular factors that regulate nuclear size during development, we developed a novel nuclear resizing assay wherein nuclei assembled in Xenopus egg extract become smaller in the presence of cytoplasmic interphase extract isolated from post-gastrula Xenopus embryos. We show that nuclear shrinkage depends on conventional protein kinase C (cPKC). Increased nuclear cPKC localization and activity and decreased nuclear association of lamins mediate nuclear size reductions during development, and manipulating cPKC activity in vivo during interphase alters nuclear size in the embryo. We propose a model of steady-state nuclear size regulation whereby nuclear expansion is balanced by an active cPKC-dependent mechanism that reduces nuclear size.

PubMed ID: 25135933
PMC ID: PMC4137061
Article link: J Cell Biol
Grant support: [+]
Genes referenced: lmnb1 lmnb3 prkca prkcb ran
Morpholinos: prkca MO1 prkcb MO1


Article Images: [+] show captions
References [+] :
Anderson, The life cycle of the metazoan nuclear envelope. 2008, Pubmed


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