Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Br J Pharmacol
2014 Dec 01;17123:5182-94. doi: 10.1111/bph.12596.
Show Gene links
Show Anatomy links
Modulation of K2P 2.1 and K2P 10.1 K(+) channel sensitivity to carvedilol by alternative mRNA translation initiation.
Kisselbach J
,
Seyler C
,
Schweizer PA
,
Gerstberger R
,
Becker R
,
Katus HA
,
Thomas D
.
???displayArticle.abstract???
BACKGROUND AND PURPOSE: The β-receptor antagonist carvedilol blocks a range of ion channels. K2P 2.1 (TREK1) and K2P 10.1 (TREK2) channels are expressed in the heart and regulated by alternative translation initiation (ATI) of their mRNA, producing functionally distinct channel variants. The first objective was to investigate acute effects of carvedilol on human K2P 2.1 and K2P 10.1 channels. Second, we sought to study ATI-dependent modulation of K2P K(+) current sensitivity to carvedilol.
EXPERIMENTAL APPROACH: Using standard electrophysiological techniques, we recorded currents from wild-type and mutant K2P 2.1 and K2P 10.1 channels in Xenopus oocytes and HEK 293 cells.
KEY RESULTS: Carvedilol concentration-dependently inhibited K2P 2.1 channels (IC50 ,oocytes = 20.3 μM; IC50 , HEK = 1.6 μM) and this inhibition was frequency-independent. When K2P 2.1 isoforms generated by ATI were studied separately in oocytes, the IC50 value for carvedilol inhibition of full-length channels (16.5 μM) was almost 5-fold less than that for the truncated channel variant (IC50 = 79.0 μM). Similarly, the related K2P 10.1 channels were blocked by carvedilol (IC50 ,oocytes = 24.0 μM; IC50 , HEK = 7.6 μM) and subject to ATI-dependent modulation of drug sensitivity.
CONCLUSIONS AND IMPLICATIONS: Carvedilol targets K2P 2.1 and K2P 10.1 K(+) channels. This previously unrecognized mechanism supports a general role of cardiac K2P channels as antiarrhythmic drug targets. Furthermore, the work reveals that the sensitivity of the cardiac ion channels K2P 2.1 and K2P 10.1 to block was modulated by alternative mRNA translation initiation.
Abi-Gerges,
hERG subunit composition determines differential drug sensitivity.
2011, Pubmed
Abi-Gerges,
hERG subunit composition determines differential drug sensitivity.
2011,
Pubmed
Acikel,
Comparison of the efficacy of metoprolol and carvedilol for preventing atrial fibrillation after coronary bypass surgery.
2008,
Pubmed
Aimond,
Simultaneous activation of p38 MAPK and p42/44 MAPK by ATP stimulates the K+ current ITREK in cardiomyocytes.
2000,
Pubmed
Alexander,
The Concise Guide to PHARMACOLOGY 2013/14: ion channels.
2013,
Pubmed
Bockenhauer,
KCNK2: reversible conversion of a hippocampal potassium leak into a voltage-dependent channel.
2001,
Pubmed
,
Xenbase
Brohawn,
Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel.
2012,
Pubmed
Brunvand,
Carvedilol improves function and reduces infarct size in the feline myocardium by protecting against lethal reperfusion injury.
1996,
Pubmed
Cheng,
Carvedilol blocks the repolarizing K+ currents and the L-type Ca2+ current in rabbit ventricular myocytes.
1999,
Pubmed
Cice,
Efficacy of carvedilol on complex ventricular arrhythmias in dilated cardiomyopathy: double-blind, randomized, placebo-controlled study.
2000,
Pubmed
Decher,
Knock-out of the potassium channel TASK-1 leads to a prolonged QT interval and a disturbed QRS complex.
2011,
Pubmed
Deng,
Effects of carvedilol on transient outward and ultra-rapid delayed rectifier potassium currents in human atrial myocytes.
2007,
Pubmed
Donner,
Functional role of TASK-1 in the heart: studies in TASK-1-deficient mice show prolonged cardiac repolarization and reduced heart rate variability.
2011,
Pubmed
Eckert,
TREK-1 isoforms generated by alternative translation initiation display different susceptibility to the antidepressant fluoxetine.
2011,
Pubmed
,
Xenbase
Fink,
Cloning, functional expression and brain localization of a novel unconventional outward rectifier K+ channel.
1996,
Pubmed
,
Xenbase
Gehr,
The pharmacokinetics of carvedilol and its metabolites after single and multiple dose oral administration in patients with hypertension and renal insufficiency.
1999,
Pubmed
Gierten,
Regulation of two-pore-domain (K2P) potassium leak channels by the tyrosine kinase inhibitor genistein.
2008,
Pubmed
,
Xenbase
Gierten,
The human cardiac K2P3.1 (TASK-1) potassium leak channel is a molecular target for the class III antiarrhythmic drug amiodarone.
2010,
Pubmed
,
Xenbase
Gierten,
Identification and functional characterization of zebrafish K(2P)10.1 (TREK2) two-pore-domain K(+) channels.
2012,
Pubmed
,
Xenbase
Goldstein,
Potassium leak channels and the KCNK family of two-P-domain subunits.
2001,
Pubmed
Goonetilleke,
TREK-1 K(+) channels in the cardiovascular system: their significance and potential as a therapeutic target.
2012,
Pubmed
Gu,
Expression pattern and functional characteristics of two novel splice variants of the two-pore-domain potassium channel TREK-2.
2002,
Pubmed
Haghjoo,
Optimal beta-blocker for prevention of atrial fibrillation after on-pump coronary artery bypass graft surgery: carvedilol versus metoprolol.
2007,
Pubmed
Honoré,
An intracellular proton sensor commands lipid- and mechano-gating of the K(+) channel TREK-1.
2002,
Pubmed
Honoré,
The neuronal background K2P channels: focus on TREK1.
2007,
Pubmed
Kanoupakis,
Electrophysiological effects of carvedilol administration in patients with dilated cardiomyopathy.
2008,
Pubmed
Karle,
Antiarrhythmic drug carvedilol inhibits HERG potassium channels.
2001,
Pubmed
,
Xenbase
Kathöfer,
Functional coupling of human beta 3-adrenoreceptors to the KvLQT1/MinK potassium channel.
2000,
Pubmed
,
Xenbase
Katritsis,
Comparison of effectiveness of carvedilol versus bisoprolol for maintenance of sinus rhythm after cardioversion of persistent atrial fibrillation.
2003,
Pubmed
Kawakami,
Comparison of HERG channel blocking effects of various beta-blockers-- implication for clinical strategy.
2006,
Pubmed
Kikuta,
Carvedilol blocks cardiac KATP and KG but not IK1 channels by acting at the bundle-crossing regions.
2006,
Pubmed
Kilkenny,
Animal research: reporting in vivo experiments: the ARRIVE guidelines.
2010,
Pubmed
Kisselbach,
Enhancement of K2P2.1 (TREK1) background currents expressed in Xenopus oocytes by voltage-gated K+ channel β subunits.
2012,
Pubmed
,
Xenbase
Limberg,
TASK-1 channels may modulate action potential duration of human atrial cardiomyocytes.
2011,
Pubmed
,
Xenbase
Liu,
Heterogeneous expression of tandem-pore K+ channel genes in adult and embryonic rat heart quantified by real-time polymerase chain reaction.
2004,
Pubmed
McGrath,
Guidelines for reporting experiments involving animals: the ARRIVE guidelines.
2010,
Pubmed
McPhillips,
Effects of carvedilol on blood pressure in patients with mild to moderate hypertension. A dose response study.
1988,
Pubmed
Medhurst,
Distribution analysis of human two pore domain potassium channels in tissues of the central nervous system and periphery.
2001,
Pubmed
Merritt,
Comparison of effectiveness of carvedilol versus metoprolol or atenolol for atrial fibrillation appearing after coronary artery bypass grafting or cardiac valve operation.
2003,
Pubmed
Miller,
Crystal structure of the human two-pore domain potassium channel K2P1.
2012,
Pubmed
Morgan,
Pharmacokinetics of carvedilol in older and younger patients.
1990,
Pubmed
Murbartián,
Sequential phosphorylation mediates receptor- and kinase-induced inhibition of TREK-1 background potassium channels.
2005,
Pubmed
Petric,
In vivo electrophysiological characterization of TASK-1 deficient mice.
2012,
Pubmed
Piechotta,
The pore structure and gating mechanism of K2P channels.
2011,
Pubmed
Poole-Wilson,
Comparison of carvedilol and metoprolol on clinical outcomes in patients with chronic heart failure in the Carvedilol Or Metoprolol European Trial (COMET): randomised controlled trial.
2003,
Pubmed
Putzke,
The acid-sensitive potassium channel TASK-1 in rat cardiac muscle.
2007,
Pubmed
,
Xenbase
Rahm,
PKC-dependent activation of human K(2P) 18.1 K(+) channels.
2012,
Pubmed
,
Xenbase
Rahm,
Functional characterization of zebrafish K2P18.1 (TRESK) two-pore-domain K+ channels.
2014,
Pubmed
,
Xenbase
Ramaswamy,
Beta blockers improve outcome in patients with heart failure and atrial fibrillation: U.S. carvedilol study.
2003,
Pubmed
Ravens,
Novel pharmacological approaches for antiarrhythmic therapy.
2010,
Pubmed
Remme,
Carvedilol protects better against vascular events than metoprolol in heart failure: results from COMET.
2007,
Pubmed
Reyes,
Cloning and expression of a novel pH-sensitive two pore domain K+ channel from human kidney.
1998,
Pubmed
Sandoz,
Optical probing of a dynamic membrane interaction that regulates the TREK1 channel.
2011,
Pubmed
,
Xenbase
Schmidt,
Novel electrophysiological properties of dronedarone: inhibition of human cardiac two-pore-domain potassium (K2P) channels.
2012,
Pubmed
,
Xenbase
Schmidt,
Cardiac expression and atrial fibrillation-associated remodeling of K₂p2.1 (TREK-1) K⁺ channels in a porcine model.
2014,
Pubmed
,
Xenbase
Senior,
Effects of carvedilol on ventricular arrhythmias.
1992,
Pubmed
Seyler,
TASK1 (K(2P)3.1) K(+) channel inhibition by endothelin-1 is mediated through Rho kinase-dependent phosphorylation.
2012,
Pubmed
,
Xenbase
Simkin,
Control of the single channel conductance of K2P10.1 (TREK-2) by the amino-terminus: role of alternative translation initiation.
2008,
Pubmed
Staudacher,
Carvedilol targets human K2P 3.1 (TASK1) K+ leak channels.
2011,
Pubmed
,
Xenbase
Staudacher,
Alternative splicing determines mRNA translation initiation and function of human K(2P)10.1 K+ channels.
2011,
Pubmed
,
Xenbase
Takusagawa,
The inhibitory effects of carvedilol against arrhythmias induced by coronary reperfusion in anesthetized rats.
2000,
Pubmed
Tan,
Differential expression of the mechanosensitive potassium channel TREK-1 in epicardial and endocardial myocytes in rat ventricle.
2004,
Pubmed
Terrenoire,
A TREK-1-like potassium channel in atrial cells inhibited by beta-adrenergic stimulation and activated by volatile anesthetics.
2001,
Pubmed
Thomas,
Alternative translation initiation in rat brain yields K2P2.1 potassium channels permeable to sodium.
2008,
Pubmed
,
Xenbase
Veale,
Dominant negative effects of a non-conducting TREK1 splice variant expressed in brain.
2010,
Pubmed
Xian Tao Li,
The stretch-activated potassium channel TREK-1 in rat cardiac ventricular muscle.
2006,
Pubmed
Yokoyama,
Electrophysiological effects of carvedilol on rabbit heart pacemaker cells.
2007,
Pubmed
Zhang,
Temperature-sensitive TREK currents contribute to setting the resting membrane potential in embryonic atrial myocytes.
2008,
Pubmed
Zhao,
Regional differential expression of TREK-1 at left ventricle in myocardial infarction.
2011,
Pubmed
Zhou,
Carvedilol and its new analogs suppress arrhythmogenic store overload-induced Ca2+ release.
2011,
Pubmed