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XB-ART-49502
Cell Dev Biol February 15, 2014; 3 (1):

Embryonic Expression and Function of the Xenopus Ink4d Cyclin D-Dependent Kinase Inhibitor.

Doherty JR , Nilsson LM , Kuliyev E , Zhu H , Matthew R , Cleveland JL , Mead PE , Roussel MF .


Abstract
Here we report the cloning and functional characterization of the cyclin D-dependent kinase 4 and 6 (Cdk4/6) inhibitory protein Cdkn2d/p19(Ink4d) of Xenopuslaevis (Xl-Ink4d). Xl-Ink4d is the only Ink4 family gene highly expressed during Xenopus development and its transcripts were detected maternally and during neurulation. The Xl-Ink4d protein has 63% identity to mouse and human Cdkn2d/p19(Ink4d) and its function as a negative regulator of cell cycle traverse is evolutionary conserved. Indeed, Xl-lnk4d can functionally substitute for mouse Cdkn2d in binding to mouse Cdk4 and inhibiting cyclin-D1-dependent CDK4 kinase activity. Further, enforced expression of Xl-lnk4d arrests mouse fibroblasts in the G1 phase of the cell cycle. These findings indicate that CDKN2d/p19(Ink4d) is conserved through vertebrate evolution and suggest Xl-lnk4d may contribute to the development of Xenopuslaevis.

PubMed ID: 25309971
PMC ID: PMC4192657
Article link: Cell Dev Biol
Grant support: [+]
Genes referenced: ank1 ccnd1 cdk4 cdk6 cdkn2d odc1
Antibodies: Cdkn2d Ab1


Article Images: [+] show captions
References:
Chen, 2003, Pubmed [+]


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