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XB-ART-49534
Nat Cell Biol October 1, 2009; 11 (10): 1225-32.

The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.

Gray RS , Abitua PB , Wlodarczyk BJ , Szabo-Rogers HL , Blanchard O , Lee I , Weiss GS , Liu KJ , Marcotte EM , Wallingford JB , Finnell RH .


Abstract
The planar cell polarity (PCP) signalling pathway is essential for embryonic development because it governs diverse cellular behaviours, and ''core PCP'' proteins, such as Dishevelled and Frizzled, have been extensively characterized. By contrast, the ''PCP effector'' proteins, such as Intu and Fuz, remain largely unstudied. These proteins are essential for PCP signalling, but they have never been investigated in mammals and their cell biological activities remain entirely unknown. We report here that Fuz mutant mice show neural tube defects, skeletal dysmorphologies and Hedgehog signalling defects stemming from disrupted ciliogenesis. Using bioinformatics and imaging of an in vivo mucociliary epithelium, we established a central role for Fuz in membrane trafficking, showing that Fuz is essential for trafficking of cargo to basal bodies and to the apical tips of cilia. Fuz is also essential for exocytosis in secretory cells. Finally, we identified a Rab-related small GTPase as a Fuz interaction partner that is also essential for ciliogenesis and secretion. These results are significant because they provide new insights into the mechanisms by which developmental regulatory systems such as PCP signalling interface with fundamental cellular systems such as the vesicle trafficking machinery.

PubMed ID: 19767740
PMC ID: PMC2755648
Article link: Nat Cell Biol

Genes referenced: cplane2 dvl2 fuz fzd7 hist2h2be intu itln1 nucb1 shh
Morpholinos: fuz MO1


Article Images: [+] show captions
References:
Ansley, 2003, Pubmed [+]


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