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XB-ART-49633
Development December 1, 2014; 141 (23): 4569-79.

An adhesome comprising laminin, dystroglycan and myosin IIA is required during notochord development in Xenopus laevis.

Buisson N , Sirour C , Moreau N , Denker E , Le Bouffant R , Goullancourt A , Darribère T , Bello V .


Abstract
Dystroglycan (Dg) is a transmembrane receptor for laminin that must be expressed at the right time and place in order to be involved in notochord morphogenesis. The function of Dg was examined in Xenopus laevis embryos by knockdown of Dg and overexpression and replacement of the endogenous Dg with a mutated form of the protein. This analysis revealed that Dg is required for correct laminin assembly, for cell polarization during mediolateral intercalation and for proper differentiation of vacuoles. Using mutations in the cytoplasmic domain, we identified two sites that are involved in cell polarization and are required for mediolateral cell intercalation, and a site that is required for vacuolation. Furthermore, using a proteomic analysis, the cytoskeletal non-muscle myosin IIA has been identified for the first time as a molecular link between the Dg-cytoplasmic domain and cortical actin. The data allowed us to identify the adhesome laminin-Dg-myosin IIA as being required to maintain the cortical actin cytoskeleton network during vacuolation, which is crucial to maintain the shape of notochordal cells.

PubMed ID: 25359726
Article link: Development

Genes referenced: acta1 chrd.1 dag1 eng fbn1 fbn2 fn1 hopx lama1 myh10 myh9 tuba4b
Antibodies: Acta1 Ab4 Dag1 Ab2 Dag1 Ab4 Fbn2 Ab1 Fn1 Ab1 GFP Ab15 GFP Ab17 Lama1 Ab1 Myh9 Ab1 Notochord Ab1 Tuba4b Ab2
Morpholinos: dag1 MO1 dag1 MO2 myh9 MO1


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