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XB-ART-49634
Development December 1, 2014; 141 (23): 4537-47.

Genome-wide view of TGFβ/Foxh1 regulation of the early mesendoderm program.

Chiu WT , Charney Le R , Blitz IL , Fish MB , Li Y , Biesinger J , Xie X , Cho KW .


Abstract
Nodal/TGFβ signaling regulates diverse biological responses. By combining RNA-seq on Foxh1 and Nodal signaling loss-of-function embryos with ChIP-seq of Foxh1 and Smad2/3, we report a comprehensive genome-wide interaction between Foxh1 and Smad2/3 in mediating Nodal signaling during vertebrate mesendoderm development. This study significantly increases the total number of Nodal target genes regulated by Foxh1 and Smad2/3, and reinforces the notion that Foxh1-Smad2/3-mediated Nodal signaling directly coordinates the expression of a cohort of genes involved in the control of gene transcription, signaling pathway modulation and tissue morphogenesis during gastrulation. We also show that Foxh1 may function independently of Nodal signaling, in addition to its role as a transcription factor mediating Nodal signaling via Smad2/3. Finally, we propose an evolutionarily conserved interaction between Foxh1 and PouV, a mechanism observed in Pou5f1-mediated regulation of pluripotency in human embryonic stem and epiblast cells.

PubMed ID: 25359723
PMC ID: PMC4302925
Article link: Development
Grant support: [+]
Genes referenced: agap1 atp1b2 bix1.1 cer1 chrd.1 ctnnb1 cyp27c1 foxa4 foxh1 foxh1.2 gata4 gata6 gsc hes7.2 hhex ins itpka lefty mcidas mespb mex3c mix1 mixer nodal nodal1 nodal2 nodal3.1 nodal3.2 nodal3.3 nodal3.4 odc1 otx2 pcdh8.2 pias4 pitx1 pitx2 pkdcc.1 pkdcc.2 pnhd pou5f3.1 pou5f3.2 pou5f3.3 rasef rpl11 sia1 smad1 smad2 smad3 snai1 sox17a sox3 ssh1 tbx2 tbxt tcf12 tfap2a uspl1 vegt ventx2.1 ventx2.2 wnt11b
Antibodies: Ctnnb1 Ab9 FLAG Ab1 FLAG Ab3 Foxh1 Ab2 Smad1 Ab11 Smad1 Ab5 Smad2 Ab4 Smad2 Ab8
Morpholinos: foxh1 MO2 pou5f3.1 MO4 pou5f3.2 MO6 pou5f3.3 MO6

GEO Series:
   GSE53652: Xenbase,  NCBI
   GSE53653: Xenbase,  NCBI
   GSE53654: NCBI


Article Images: [+] show captions
References [+] :
Agius, Endodermal Nodal-related signals and mesoderm induction in Xenopus. 2000, Pubmed, Xenbase


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