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Proc Biol Sci
2014 Dec 07;2811796:20141838. doi: 10.1098/rspb.2014.1838.
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Vitamin supplementation by gut symbionts ensures metabolic homeostasis in an insect host.
Salem H
,
Bauer E
,
Strauss AS
,
Vogel H
,
Marz M
,
Kaltenpoth M
.
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Despite the demonstrated functional importance of gut microbes, our understanding of how animals regulate their metabolism in response to nutritionally beneficial symbionts remains limited. Here, we elucidate the functional importance of the African cotton stainer's (Dysdercus fasciatus) association with two actinobacterial gut symbionts and subsequently examine the insect's transcriptional response following symbiont elimination. In line with bioassays demonstrating the symbionts' contribution towards host fitness through the supplementation of B vitamins, comparative transcriptomic analyses of genes involved in import and processing of B vitamins revealed an upregulation of gene expression in aposymbiotic (symbiont-free) compared with symbiotic individuals; an expression pattern that is indicative of B vitamin deficiency in animals. Normal expression levels of these genes, however, can be restored by either artificial supplementation of B vitamins into the insect's diet or reinfection with the actinobacterial symbionts. Furthermore, the functional characterization of the differentially expressed thiamine transporter 2 through heterologous expression in Xenopus laevis oocytes confirms its role in cellular uptake of vitamin B1. These findings demonstrate that despite an extracellular localization, beneficial gut microbes can be integral to the host's metabolic homeostasis, reminiscent of bacteriome-localized intracellular mutualists.
Abbas,
Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
2011, Pubmed
Abbas,
Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
2011,
Pubmed
Akman,
Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia.
2002,
Pubmed
Bauer,
Transcriptomic immune response of the cotton stainer Dysdercus fasciatus to experimental elimination of vitamin-supplementing intestinal symbionts.
2014,
Pubmed
Beard,
Bacterial symbionts of the triatominae and their potential use in control of Chagas disease transmission.
2002,
Pubmed
Daffre,
The lysozyme locus in Drosophila melanogaster: an expanded gene family adapted for expression in the digestive tract.
1994,
Pubmed
Douglas,
Phloem-sap feeding by animals: problems and solutions.
2006,
Pubmed
Feldhaar,
Nutritional upgrading for omnivorous carpenter ants by the endosymbiont Blochmannia.
2007,
Pubmed
Fukatsu,
Capsule-transmitted gut symbiotic bacterium of the Japanese common plataspid stinkbug, Megacopta punctatissima.
2002,
Pubmed
Hansen,
Aphid genome expression reveals host-symbiont cooperation in the production of amino acids.
2011,
Pubmed
Hohmann,
Thiamin metabolism and thiamin diphosphate-dependent enzymes in the yeast Saccharomyces cerevisiae: genetic regulation.
1998,
Pubmed
Hosokawa,
Wolbachia as a bacteriocyte-associated nutritional mutualist.
2010,
Pubmed
Ink,
Vitamin B6 metabolism.
1984,
Pubmed
Jackowski,
Regulation of coenzyme A biosynthesis.
1981,
Pubmed
Kaltenpoth,
Localization and transmission route of Coriobacterium glomerans, the endosymbiont of pyrrhocorid bugs.
2009,
Pubmed
Kanehisa,
KEGG for integration and interpretation of large-scale molecular data sets.
2012,
Pubmed
Kikuchi,
Insect-microbe mutualism without vertical transmission: a stinkbug acquires a beneficial gut symbiont from the environment every generation.
2007,
Pubmed
Kim,
Specific midgut region controlling the symbiont population in an insect-microbe gut symbiotic association.
2013,
Pubmed
Kostál,
The 70 kDa heat shock protein assists during the repair of chilling injury in the insect, Pyrrhocoris apterus.
2009,
Pubmed
Moran,
Symbiosis as an adaptive process and source of phenotypic complexity.
2007,
Pubmed
Nakabachi,
Transcriptome analysis of the aphid bacteriocyte, the symbiotic host cell that harbors an endocellular mutualistic bacterium, Buchnera.
2005,
Pubmed
Payne,
Retention and loss of amino acid biosynthetic pathways based on analysis of whole-genome sequences.
2006,
Pubmed
Piroli,
Biphasic effects of stress upon GLUT8 glucose transporter expression and trafficking in the diabetic rat hippocampus.
2004,
Pubmed
Price,
Aphid amino acid transporter regulates glutamine supply to intracellular bacterial symbionts.
2014,
Pubmed
,
Xenbase
Qiu,
Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction.
2007,
Pubmed
,
Xenbase
Ribeil,
Hsp70 regulates erythropoiesis by preventing caspase-3-mediated cleavage of GATA-1.
2007,
Pubmed
Said,
Uptake of biotin by human hepatoma cell line, Hep G2: a carrier-mediated process similar to that of normal liver.
1994,
Pubmed
Said,
Mechanism of nicotinic acid transport in human liver cells: experiments with HepG2 cells and primary hepatocytes.
2007,
Pubmed
Salem,
Actinobacteria as essential symbionts in firebugs and cotton stainers (Hemiptera, Pyrrhocoridae).
2013,
Pubmed
Stackebrandt,
Complete genome sequence of Coriobacterium glomerans type strain (PW2(T)) from the midgut of Pyrrhocoris apterus L. (red soldier bug).
2013,
Pubmed
Strauss,
ABC transporter functions as a pacemaker for sequestration of plant glucosides in leaf beetles.
2013,
Pubmed
Sudakaran,
Geographical and ecological stability of the symbiotic mid-gut microbiota in European firebugs, Pyrrhocoris apterus (Hemiptera, Pyrrhocoridae).
2012,
Pubmed
Zhao,
Mechanisms of membrane transport of folates into cells and across epithelia.
2011,
Pubmed
de Crécy-Lagard,
Comparative genomics of bacterial and plant folate synthesis and salvage: predictions and validations.
2007,
Pubmed