On Wednesday Feb. 22, 2017 Xenbase will be in a maintenance phase. The site will be available but some functions may be disabled.

Click on this message to dismiss it.
Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Development. September 1, 2003; 130 (17): 4047-56.

The pro-BMP activity of Twisted gastrulation is independent of BMP binding.

Oelgeschläger M , Reversade B , Larraín J , Little S , Mullins MC , De Robertis EM .

The determination of the vertebrate dorsoventral body axis is regulated in the extracellular space by a system of interacting secreted molecules consisting of BMP, Chordin, Tolloid and Twisted Gastrulation (Tsg). Tsg is a BMP-binding protein that forms ternary complexes with BMP and Chordin. We investigated the function of Tsg in embryonic patterning by generating point mutations in its two conserved cysteine-rich domains. Surprisingly, Tsg proteins with mutations in the N-terminal domain were unable to bind BMP, yet ventralized the embryo very effectively, indicating strong pro-BMP activity. This hyperventralizing Tsg activity required an intact C-terminal domain and could block the anti-BMP activity of isolated BMP-binding modules of Chordin (CRs) in embryonic assays. This activity was specific for CR-containing proteins as it did not affect the dorsalizing effects of Noggin or dominant-negative BMP receptor. The ventralizing effects of the xTsg mutants were stronger than the effect of Chordin loss-of-function in Xenopus or zebrafish. The results suggest that xTsg interacts with additional CR-containing proteins that regulate dorsoventral development in embryos.

PubMed ID: 12874126
PMC ID: PMC2277362
Article link: Development.
Grant support: GM 56326 NIGMS NIH HHS , HD 21502-17 NICHD NIH HHS , R37 HD021502-17 NICHD NIH HHS , R37 HD021502 NICHD NIH HHS

Genes referenced: bmp1 bmp4 chrd.1 myod1 nog pclo pmp22 sox2 tbx2 tdgf1.3 tll2 tubb2b twsg1
Morpholinos referenced: chrd MO1 chrd MO2

Resources URL:

Article Images: [+] show captions

My Xenbase: [ Log-in / Register ]
version: [4.2.0]

Major funding for Xenbase is provided by the National Institute of Child Health and Human Development, grant P41 HD064556