XB-ART-49760Neuron June 4, 2014; 82 (5): 1004-16.
Non-cell-autonomous mechanism of activity-dependent neurotransmitter switching.
Activity-dependent neurotransmitter switching engages genetic programs regulating transmitter synthesis, but the mechanism by which activity is transduced is unknown. We suppressed activity in single neurons in the embryonic spinal cord to determine whether glutamate-gamma-aminobutyric acid (GABA) switching is cell autonomous. Transmitter respecification did not occur, suggesting that it is homeostatically regulated by the level of activity in surrounding neurons. Graded increase in the number of silenced neurons in cultures led to graded decrease in the number of neurons expressing GABA, supporting non-cell-autonomous transmitter switching. We found that brain-derived neurotrophic factor (BDNF) is expressed in the spinal cord during the period of transmitter respecification and that spike activity causes release of BDNF. Activation of TrkB receptors triggers a signaling cascade involving JNK-mediated activation of cJun that regulates tlx3, a glutamate/GABA selector gene, accounting for calcium-spike BDNF-dependent transmitter switching. Our findings identify a molecular mechanism for activity-dependent respecification of neurotransmitter phenotype in developing spinal neurons.
PubMed ID: 24908484
PMC ID: PMC4072120
Article link: Neuron
Genes referenced: bdnf mapk8 ntrk2 tlx3
References [+] :
Abe, Postnatal development of neurons, interneurons and glial cells in the substantia nigra of mice. 2010, Pubmed