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XB-ART-49800
Nucleic Acids Res June 1, 2014; 42 (10): 6645-58.
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HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment.

Taniguchi I , Mabuchi N , Ohno M .


Abstract
Nuclear RNA export pathways in eukaryotes are often linked to the fate of a given RNA. Therefore, the choice of export pathway should be well-controlled to avoid an unfavorable effect on gene expression. Although some RNAs could be exported by more than one pathway, little is known about how the choice is regulated. This issue is highlighted when the human immunodeficiency virus type 1 (HIV-1) Rev protein induces the export of singly spliced and unspliced HIV-1 transcripts. How these RNAs are exported is not well understood because such transcripts should have the possibility of utilizing CRM1-dependent export via Rev or cellular TAP/NXF1-dependent export via the transcription/export (TREX) complex, or both. Here we found that Rev suppressed TAP/NXF1-dependent export of model RNA substrates that recapitulated viral transcripts. In this effect, Rev interacted with the cap-binding complex and inhibited the recruitment of the TREX complex. Thus, Rev controls the identity of the factor occupying the cap-proximal region that determines the RNA export pathway. This ribonucleoprotein remodeling activity of Rev may favor viral gene expression.

PubMed ID: 24753416
PMC ID: PMC4041468
Article link: Nucleic Acids Res


Species referenced: Xenopus
Genes referenced: alyref cdkn2b ddx39b hnrnpa1 hnrnpc mmut myc ncbp1 nxf1 rbm8a u2af2 xpo1


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References [+] :
Arnold, Multiple importins function as nuclear transport receptors for the Rev protein of human immunodeficiency virus type 1. 2006, Pubmed