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J Cell Biol March 17, 2014; 204 (6): 947-63.

EB1 enables spindle microtubules to regulate centromeric recruitment of Aurora B.

Banerjee B , Kestner CA , Stukenberg PT .

The Aurora B kinase coordinates kinetochore-microtubule attachments with spindle checkpoint signaling on each mitotic chromosome. We find that EB1, a microtubule plus end-tracking protein, is required to enrich Aurora B at inner centromeres in a microtubule-dependent manner. This regulates phosphorylation of both kinetochore and chromatin substrates. EB1 regulates the histone phosphorylation marks (histone H2A phospho-Thr120 and histone H3 phospho-Thr3) that localize Aurora B. The chromosomal passenger complex containing Aurora B can be found on a subset of spindle microtubules that exist near prometaphase kinetochores, known as preformed K-fibers (kinetochore fibers). Our data suggest that EB1 enables the spindle microtubules to regulate the phosphorylation of kinetochores through recruitment of the Aurora B kinase.

PubMed ID: 24616220
PMC ID: PMC3998792
Article link: J Cell Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: aurkb birc5 bub1 cdca8 h2ac21 hoxc6 incenp kidins220 knl1 mapre1 mbp myc ndc80 noct tbx2 tub

Article Images: [+] show captions
References [+] :
Bayliss, Structural basis of Aurora-A activation by TPX2 at the mitotic spindle. 2003, Pubmed, Xenbase