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Development. January 1, 2015; 142 (1): 99-107.

Vangl2 cooperates with Rab11 and Myosin V to regulate apical constriction during vertebrate gastrulation.

Ossipova O , Chuykin I , Chu CW , Sokol SY .

Core planar cell polarity (PCP) proteins are well known to regulate polarity in Drosophila and vertebrate epithelia; however, their functions in vertebrate morphogenesis remain poorly understood. In this study, we describe a role for PCP signaling in the process of apical constriction during Xenopus gastrulation. The core PCP protein Vangl2 is detected at the apical surfaces of cells at the blastopore lip, and it functions during blastopore formation and closure. Further experiments show that Vangl2, as well as Daam1 and Rho-associated kinase (Rock), regulate apical constriction of bottle cells at the blastopore and ectopic constriction of ectoderm cells triggered by the actin-binding protein Shroom3. At the blastopore lip, Vangl2 is required for the apical accumulation of the recycling endosome marker Rab11. We also show that Rab11 and the associated motor protein Myosin V play essential roles in both endogenous and ectopic apical constriction, and might be involved in Vangl2 trafficking to the cell surface. Overexpression of Rab11 RNA was sufficient to partly restore normal blastopore formation in Vangl2-deficient embryos. These observations suggest that Vangl2 affects Rab11 to regulate apical constriction during blastopore formation.

PubMed ID: 25480917
PMC ID: PMC4299144
Article link: Development.
Grant support: HD078874 NICHD NIH HHS , NS40972 NINDS NIH HHS , R01 NS040972 NINDS NIH HHS , R21 HD078874 NICHD NIH HHS , R56 NS040972 NINDS NIH HHS

Genes referenced: actl6a daam1 itgb1 myl2 myo5b rab11a rhoa rock1 shroom3 vangl2

Antibodies referenced: Itgb1 Ab1 Phosphorylated myosin light chain Ab1 Rab11a Ab2 Rab11a Ab3 Rab11a Ab4 Vangl2 Ab2 Vangl2 Ab3
Morpholinos referenced: daam1 MO2 rab11a MO1 vangl2 MO2

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Agius, 2000, Pubmed, Xenbase [+]

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